Delta 1 Catenin/P120 Catenin (CTNND1) Antibody – Rabbit Monoclonal

Western blot analysis of delta1 Catenin expression in HeLa cell lysate (MBS179829).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNND1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTNND1

All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.

Immunofluorescent analysis of MCF-7 cells, using delta 1 Catenin/p120 Catenin Antibody

All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.

Western blot analysis of delta1 Catenin expression in HeLa cell lysate.