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VAMP8 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179830

SNAREs, soluble N-ethylmaleimide-sensitive factor- attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
VAMP8/Endobrevin, Monoclonal Antibody
Full Product Name
Anti-VAMP8/Endobrevin Rabbit Monoclonal Antibody
Product Gene Name
anti-VAMP8 antibody
Product Synonym Names
Vesicle-associated membrane protein 8; VAMP-8; Endobrevin; EDB; VAMP8
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
11438 MW
OMIM
AF053233 mRNA
3D Structure
ModBase 3D Structure for Q9BV40
Immunogen
A synthesized peptide derived from human VAMP8
Subcellular Localization
Lysosome membrane; Single-pass type IV membrane protein. Early endosome membrane; Single-pass type IV membrane protein. Late endosome membrane; Single-pass type IV membrane protein. Cell membrane; Single-pass type IV membrane protein. Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (By similarity). Sub-tight junctional domain in retinal pigment epithelium cells. Midbody region during cytokinesis. Lumenal oriented, apical membranes of nephric tubular cell (By similarity). Cycles through the apical but not through the basolateral plasma membrane (By similarity). Apical region of acinar cells; in zymogen granule membranes (By similarity).
Tissue Specificity
Platelets.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-VAMP8 antibody

Neuroscience; Neurotransmission; Secretory Vesicles; Snaps & Snares; Immunology; Innate Immunity; Macrophage/Inflamm.; Neuroscience; Cell Type Marker; neuron marker; synapse marker
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-VAMP8 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-VAMP8 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50

Western Blot (WB) of anti-VAMP8 antibody

Western blot analysis of VAMP8 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate (MBS179830).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAMP8 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VAMP8
Rabbit Anti-VAMP8 Monoclonal Antibody – Cat# MBS179830

Other Notes

Small volumes of anti-VAMP8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VAMP8 purchase

MBS179830 is an antibody targeting VAMP8. Also known as Anti-VAMP8/Endobrevin Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VAMP8. It may not necessarily be applicable to this product.

NCBI GI #
14043026
NCBI GeneID
8673
NCBI Accession #
NP_003752.2
UniProt Primary Accession #
Q9BV40
UniProt Secondary Accession #
O60625; Q53SP9; Q6IB09
UniProt Related Accession #
Q9BV40
GenBank Nucleotide #
NM_003761.4
NCBI Official Full Name
vesicle-associated membrane protein 8
NCBI Official Synonym Full Names
vesicle associated membrane protein 8
NCBI Official Symbol
VAMP8
NCBI Official Synonym Symbols
EDB; VAMP-8
UniProt Protein Name
Vesicle-associated membrane protein 8
UniProt Synonym Protein Names
Endobrevin
Protein Family
Vesicle-associated membrane protein
UniProt Gene Name
VAMP8
UniProt Synonym Gene Names
EDB
NCBI Protein Information
vesicle-associated membrane protein 8

NCBI Summary for VAMP8

This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The encoded protein is involved in the fusion of synaptic vesicles with the presynaptic membrane.[provided by RefSeq, Jun 2010]
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UniProt Comments for VAMP8

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed:23217709, PubMed:25686604). Also required for dense-granule secretion in platelets (PubMed:12130530). Plays also a role in regulated enzyme secretion in pancreatic acinar cells (). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (). Involved in the homotypic fusion of early and late endosomes ().
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Research Articles on VAMP8

1. our study suggested that VAMP8 gene variants might not contribute to glioma susceptibility and associated with glioma in the Chinese Han population.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VAMP8 antibody

 Reference Product  PubMed Publications
 VAMP2 antibody  >27 publications with VAMP8 and VAMP2
 SNAP23 antibody  >19 publications with VAMP8 and SNAP23
 STX1A antibody  >6 publications with VAMP8 and STX1A
 STX8 antibody  >3 publications with VAMP8 and STX8
 STX3 antibody  >3 publications with VAMP8 and STX3
 STX7 antibody  >3 publications with VAMP8 and STX7
 STX2 antibody  >1 publications with VAMP8 and STX2
 STX6 antibody  >1 publications with VAMP8 and STX6
 STX4 antibody  >1 publications with VAMP8 and STX4

Pathways associated with anti-VAMP8 antibody

 Products by Pathway  Pathway Diagram
 Clathrin Derived Vesicle Budding Pathway antibodies  Clathrin Derived Vesicle Budding Pathway Diagram
 Golgi Associated Vesicle Biogenesis Pathway antibodies  Golgi Associated Vesicle Biogenesis Pathway Diagram
 Lysosome Vesicle Biogenesis Pathway antibodies  Lysosome Vesicle Biogenesis Pathway Diagram
 Membrane Trafficking Pathway antibodies  Membrane Trafficking Pathway Diagram
 Platelet Activation Pathway antibodies  Platelet Activation Pathway Diagram
 SNARE Interactions In Vesicular Transport Pathway antibodies  SNARE Interactions In Vesicular Transport Pathway Diagram
 SNARE Interactions In Vesicular Transport Pathway antibodies  SNARE Interactions In Vesicular Transport Pathway Diagram
 Trans-Golgi Network Vesicle Budding Pathway antibodies  Trans-Golgi Network Vesicle Budding Pathway Diagram

Diseases associated with anti-VAMP8 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >9 publications with VAMP8 and Inflammation
 Heart Diseases Antibodies  >6 publications with VAMP8 and Heart Diseases
 Fibrosis Antibodies  >3 publications with VAMP8 and Fibrosis
 Necrosis Antibodies  >3 publications with VAMP8 and Necrosis
 Carcinoma Antibodies  >3 publications with VAMP8 and Carcinoma
 Breast Neoplasms Antibodies  >2 publications with VAMP8 and Breast Neoplasms
 Kidney Diseases Antibodies  >2 publications with VAMP8 and Kidney Diseases
 Neoplasms, Experimental Antibodies  >2 publications with VAMP8 and Neoplasms, Experimental
 Brain Diseases Antibodies  >2 publications with VAMP8 and Brain Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with VAMP8 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-VAMP8 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >21 publications with VAMP8 and Blood
 Pancreas Antibodies  >13 publications with VAMP8 and Pancreas
 Liver Antibodies  >7 publications with VAMP8 and Liver
 Kidney Antibodies  >6 publications with VAMP8 and Kidney
 Bone Antibodies  >6 publications with VAMP8 and Bone
 Bone Marrow Antibodies  >5 publications with VAMP8 and Bone Marrow
 Eye Antibodies  >5 publications with VAMP8 and Eye
 Lung Antibodies  >3 publications with VAMP8 and Lung
 Brain Antibodies  >2 publications with VAMP8 and Brain
 Prostate Antibodies  >2 publications with VAMP8 and Prostate
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