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ACADM/MCAD Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179836

Acyl-CoA dehydrogenase specific for acyl chain lengths of 4 to 16 that catalyzes the initial step of fatty acid beta- oxidation. Utilizes the electron transfer flavoprotein (ETF) as an electron acceptor to transfer electrons to the main mitochondrial… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ACADM/Mcad, Monoclonal Antibody
Full Product Name
Anti-ACADM/Mcad Rabbit Monoclonal Antibody
Product Gene Name
anti-ACADM/Mcad antibody
Product Synonym Gene Name
ACADM
Product Synonym Names
Medium-chain specific acyl-CoA dehydrogenase; mitochondrial; MCAD; 1.3.8.7; ACADM
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
46588 MW
OMIM
M16827 mRNA
3D Structure
ModBase 3D Structure for P11310
Immunogen
A synthesized peptide derived from human ACADM
Subcellular Localization
Mitochondrion matrix.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-ACADM/Mcad antibody

Signal Transduction; Metabolism; Mitochondrial; Signal Transduction; Lipid Metabolism; Cardiovascular; Lipids/Lipoproteins; Fatty Acids; Cancer; Cancer Metabolism; Metabolic Signaling Pathway; Metabolism of Lipids and Lipoproteins; Metabolism; Pathways an
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ACADM/Mcad antibody
Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-ACADM/Mcad antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50

Western Blot (WB) of anti-ACADM/Mcad antibody

Western blot analysis of ACADM expression in (1) HeLa cell lysate; (2) K562 cell lysate (MBS179836).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACADM monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACADM
Rabbit Anti-ACADM/MCAD Monoclonal Antibody – Cat# MBS179836

Other Notes

Small volumes of anti-ACADM/Mcad antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACADM/Mcad purchase

MBS179836 is an antibody targeting ACADM/Mcad. Also known as Anti-ACADM/Mcad Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ACADM/Mcad. It may not necessarily be applicable to this product.

NCBI GI #
4557231
NCBI GeneID
34
NCBI Accession #
NP_000007.1
UniProt Primary Accession #
P11310
UniProt Secondary Accession #
Q5T4U4; Q9NYF1
UniProt Related Accession #
P11310
GenBank Nucleotide #
NM_000016.5
NCBI Official Full Name
medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform a
NCBI Official Synonym Full Names
acyl-CoA dehydrogenase medium chain
NCBI Official Symbol
ACADM
NCBI Official Synonym Symbols
MCAD; ACAD1; MCADH
UniProt Protein Name
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Gene Name
ACADM
UniProt Synonym Gene Names
MCAD
NCBI Protein Information
medium-chain specific acyl-CoA dehydrogenase, mitochondrial

NCBI Summary for ACADM/Mcad

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for ACADM/Mcad

Acyl-CoA dehydrogenase specific for acyl chain lengths of 4 to 16 that catalyzes the initial step of fatty acid beta-oxidation. Utilizes the electron transfer flavoprotein (ETF) as an electron acceptor to transfer electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
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Research Articles on ACADM/Mcad

1. Our study has revealed the unique genetic backgrounds of MCAD deficiency among Japanese, based on the largest series of non-Caucasian cases.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ACADM/Mcad antibody

 Reference Product  PubMed Publications
 ACADL antibody  >7 publications with ACADM/Mcad and ACADL
 CPT1A antibody  >3 publications with ACADM/Mcad and CPT1A
 ECHS1 antibody  >1 publications with ACADM/Mcad and ECHS1
 EHHADH antibody  >1 publications with ACADM/Mcad and EHHADH
 ACAA2 antibody  >1 publications with ACADM/Mcad and ACAA2
 HADHB antibody  >1 publications with ACADM/Mcad and HADHB

Pathways associated with anti-ACADM/Mcad antibody

 Products by Pathway  Pathway Diagram
 Beta Oxidation Of Decanoyl-CoA To Octanoyl-CoA-CoA Pathway antibodies  Beta Oxidation Of Decanoyl-CoA To Octanoyl-CoA-CoA Pathway Diagram
 Beta Oxidation Of Octanoyl-CoA To Hexanoyl-CoA Pathway antibodies  Beta Oxidation Of Octanoyl-CoA To Hexanoyl-CoA Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 Fatty Acid Beta Oxidation Pathway antibodies  Fatty Acid Beta Oxidation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram

Diseases associated with anti-ACADM/Mcad antibody

 Disease Name  Pubmed Publications
 Medium chain acyl CoA dehydrogenase deficiency Antibodies  >21 publications with ACADM/Mcad and Medium chain acyl CoA dehydrogenase deficiency
 Fatty Liver Antibodies  >12 publications with ACADM/Mcad and Fatty Liver
 Obesity Antibodies  >5 publications with ACADM/Mcad and Obesity
 Liver Diseases Antibodies  >4 publications with ACADM/Mcad and Liver Diseases
 Cardiovascular Diseases Antibodies  >4 publications with ACADM/Mcad and Cardiovascular Diseases
 Hepatomegaly Antibodies  >3 publications with ACADM/Mcad and Hepatomegaly
 Heart Diseases Antibodies  >3 publications with ACADM/Mcad and Heart Diseases
 Inflammation Antibodies  >2 publications with ACADM/Mcad and Inflammation
 Insulin Resistance Antibodies  >2 publications with ACADM/Mcad and Insulin Resistance
 Disease Models, Animal Antibodies  >2 publications with ACADM/Mcad and Disease Models, Animal

Organs/Tissues associated with anti-ACADM/Mcad antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >18 publications with ACADM/Mcad and Blood
 Liver Antibodies  >13 publications with ACADM/Mcad and Liver
 Muscle Antibodies  >5 publications with ACADM/Mcad and Muscle
 Heart Antibodies  >5 publications with ACADM/Mcad and Heart
 Adipose Tissue Antibodies  >5 publications with ACADM/Mcad and Adipose Tissue
 Connective Tissue Antibodies  >3 publications with ACADM/Mcad and Connective Tissue
 Kidney Antibodies  >3 publications with ACADM/Mcad and Kidney
 Thyroid Antibodies  >2 publications with ACADM/Mcad and Thyroid
 Vascular Antibodies  >2 publications with ACADM/Mcad and Vascular
 Pancreas Antibodies  >2 publications with ACADM/Mcad and Pancreas
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