Rabbit Anti-FTH1 Monoclonal Antibody – Cat# MBS179842

Western blot analysis of Ferritin expression in Jurkat cell lysate (MBS179842).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FTH1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FTH1

Ferritin (FTH1) Antibody – Rabbit Monoclonal

Name: FTH1 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179842
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179842

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells.… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Ferritin (FTH1), Monoclonal Antibody

Full Product Name

Anti-Ferritin Rabbit Monoclonal Antibody

Product Gene Name

anti-FTH1 antibody

Product Synonym Names

Ferritin heavy chain; Ferritin H subunit; 1.16.3.1; Cell proliferation-inducing gene 15 protein; Ferritin heavy chain; N-terminally processed; FTH1; FTH; FTHL6; OK/SW-cl.84; PIG15

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

21226 MW

OMIM

X00318 mRNA

3D Structure

ModBase 3D Structure for P02794

Immunogen

A synthesized peptide derived from human Ferritin

Tissue Specificity

Expressed in the liver.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-FTH1 antibody

Neuroscience; Neurology Process; Neurodegenerative Disease; Alzheimer's Disease; Cardiovascular; Blood; Serum Proteins; Signal Transduction; Metabolism; Vitamins/Minerals; Metabolism; Pathways and Processes; Cofactors; Vitamins/Minerals; Types of Disease

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-FTH1 antibody

Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-FTH1 antibody

WB: 1:500-1:2000
ICC/IF: 1:50-1:200

Other Notes

Small volumes of anti-FTH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FTH1 purchase

MBS179842 is an antibody targeting FTH1. Also known as Anti-Ferritin Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FTH1. It may not necessarily be applicable to this product.

NCBI GI #

56682959

NCBI GeneID

2495

NCBI Accession #

NP_002023.2

UniProt Primary Accession #

P02794

UniProt Secondary Accession #

Q3KRA8; Q3SWW1; B3KNR5

UniProt Related Accession #

P02794

GenBank Nucleotide #

NM_002032.2

NCBI Official Full Name

ferritin heavy chain

NCBI Official Synonym Full Names

ferritin heavy chain 1

NCBI Official Symbol

FTH1

NCBI Official Synonym Symbols

FHC; FTH; HFE5; PLIF; FTHL6; PIG15

UniProt Protein Name

Ferritin heavy chain

UniProt Synonym Protein Names

Cell proliferation-inducing gene 15 protein

Protein Family

Iron transporter

UniProt Gene Name

FTH1

UniProt Synonym Gene Names

FTH; FTHL6; Ferritin H subunit

NCBI Protein Information

ferritin heavy chain

NCBI Summary for FTH1

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

UniProt Comments for FTH1

Research Articles on FTH1

1. Recombinant human H-chain ferritin nanocages were characterized by transmission electron microscopy and dynamic light scattering. These results indicate that H-chain ferritin is able to self-assemble into nanocages with a narrow size distribution.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FTH1 antibody

Reference Product	PubMed Publications
FTL antibody	>9 publications with FTH1 and FTL

Pathways associated with anti-FTH1 antibody

Products by Pathway	Pathway Diagram
Clathrin Derived Vesicle Budding Pathway antibodies	Clathrin Derived Vesicle Budding Pathway Diagram
Golgi Associated Vesicle Biogenesis Pathway antibodies	Golgi Associated Vesicle Biogenesis Pathway Diagram
Iron Metabolism In Placenta Pathway antibodies	Iron Metabolism In Placenta Pathway Diagram
Iron Uptake And Transport Pathway antibodies	Iron Uptake And Transport Pathway Diagram
Membrane Trafficking Pathway antibodies	Membrane Trafficking Pathway Diagram
Mineral Absorption Pathway antibodies	Mineral Absorption Pathway Diagram
Mineral Absorption Pathway antibodies	Mineral Absorption Pathway Diagram
Porphyrin And Chlorophyll Metabolism Pathway antibodies	Porphyrin And Chlorophyll Metabolism Pathway Diagram
Porphyrin And Chlorophyll Metabolism Pathway antibodies	Porphyrin And Chlorophyll Metabolism Pathway Diagram
Transmembrane Transport Of Small Molecules Pathway antibodies	Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with anti-FTH1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>9 publications with FTH1 and Neoplasms
Inflammation Antibodies	>5 publications with FTH1 and Inflammation
Adenocarcinoma Antibodies	>3 publications with FTH1 and Adenocarcinoma
Disease Models, Animal Antibodies	>3 publications with FTH1 and Disease Models, Animal
Liver Diseases Antibodies	>2 publications with FTH1 and Liver Diseases
Nerve Degeneration Antibodies	>2 publications with FTH1 and Nerve Degeneration
Nervous System Diseases Antibodies	>2 publications with FTH1 and Nervous System Diseases
Neurodegenerative Diseases Antibodies	>2 publications with FTH1 and Neurodegenerative Diseases
Drug Toxicity Antibodies	>1 publications with FTH1 and Drug Toxicity
Edema Antibodies	>1 publications with FTH1 and Edema