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14-3-3 alpha + beta Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179852

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
14-3-3 alpha + beta, Monoclonal Antibody
Full Product Name
Anti-14-3-3 alpha + beta Rabbit Monoclonal Antibody
Product Gene Name
anti-14-3-3 alpha + beta antibody
Product Synonym Gene Name
YWHAB
Product Synonym Names
14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1; 14-3-3 protein beta/alpha, N-terminally processed; YWHAB
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
28082 MW
OMIM
X57346 mRNA
3D Structure
ModBase 3D Structure for P31946
Immunogen
A synthesized peptide derived from human 14-3-3 alpha + beta
Subcellular Localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-14-3-3 alpha + beta antibody

Neuroscience; Neurology Process; Neural Signal Transduction; Signal Transduction; Adapters; Cytoplasmic; Neuroscience; Cell Type Marker; neuron marker; synapse marker; Stem Cells; Signaling Pathways; wnt; 14-3-3
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-14-3-3 alpha + beta antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-14-3-3 alpha + beta antibody
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:30
FC/FACS: 1:30

Western Blot (WB) of anti-14-3-3 alpha + beta antibody

Western blot analysis of 14-3-3 alpha + beta expression in Hela lysate (MBS179852).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YWHAB
Rabbit Anti-14-3-3 alpha + beta Monoclonal Antibody – Cat# MBS179852

Other Notes

Small volumes of anti-14-3-3 alpha + beta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 14-3-3 alpha + beta purchase

MBS179852 is an antibody targeting 14-3-3 alpha + beta. Also known as Anti-14-3-3 alpha + beta Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for 14-3-3 alpha + beta. It may not necessarily be applicable to this product.

NCBI GI #
4507949
NCBI GeneID
7529
NCBI Accession #
NP_003395.1
UniProt Primary Accession #
P31946
UniProt Secondary Accession #
A8K9K2; E1P616
UniProt Related Accession #
P31946
GenBank Nucleotide #
NM_003404.4
NCBI Official Full Name
14-3-3 protein beta/alpha
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
NCBI Official Symbol
YWHAB
NCBI Official Synonym Symbols
HS1; GW128; YWHAA; KCIP-1; HEL-S-1
UniProt Protein Name
14-3-3 protein beta/alpha
UniProt Synonym Protein Names
Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1
Protein Family
17-beta-hydroxysteroid dehydrogenase
UniProt Gene Name
YWHAB
UniProt Synonym Gene Names
KCIP-1
NCBI Protein Information
14-3-3 protein beta/alpha

NCBI Summary for 14-3-3 alpha + beta

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for 14-3-3 alpha + beta

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
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Research Articles on 14-3-3 alpha + beta

1. Data show that 14-3-3beta protein augmented the expression of matrix metalloproteinasea MMP2 and MMP9 through PI3 kinase/Akt protein/NF-kappaappa B pathway, thereby enhancing the invasiveness of hepatocellular carcinoma (HCC) cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-14-3-3 alpha + beta antibody

 Reference Product  PubMed Publications
 CDC25B antibody  >3 publications with 14-3-3 alpha + beta and CDC25B
 CDC25A antibody  >3 publications with 14-3-3 alpha + beta and CDC25A
 IGF1R antibody  >3 publications with 14-3-3 alpha + beta and IGF1R
 CDC25C antibody  >2 publications with 14-3-3 alpha + beta and CDC25C
 YWHAE antibody  >2 publications with 14-3-3 alpha + beta and YWHAE
 SRC antibody  >2 publications with 14-3-3 alpha + beta and SRC
 KCNK3 antibody  >1 publications with 14-3-3 alpha + beta and KCNK3
 EPB41L1 antibody  >1 publications with 14-3-3 alpha + beta and EPB41L1
 BAD antibody  >1 publications with 14-3-3 alpha + beta and BAD
 TSC2 antibody  >1 publications with 14-3-3 alpha + beta and TSC2

Pathways associated with anti-14-3-3 alpha + beta antibody

 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram

Diseases associated with anti-14-3-3 alpha + beta antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >15 publications with 14-3-3 alpha + beta and Neoplasms
 Nervous System Diseases Antibodies  >11 publications with 14-3-3 alpha + beta and Nervous System Diseases
 Carcinoma Antibodies  >6 publications with 14-3-3 alpha + beta and Carcinoma
 Liver Diseases Antibodies  >3 publications with 14-3-3 alpha + beta and Liver Diseases
 Intestinal Neoplasms Antibodies  >3 publications with 14-3-3 alpha + beta and Intestinal Neoplasms
 Cardiovascular Diseases Antibodies  >2 publications with 14-3-3 alpha + beta and Cardiovascular Diseases
 Inflammation Antibodies  >2 publications with 14-3-3 alpha + beta and Inflammation
 Liver Neoplasms Antibodies  >2 publications with 14-3-3 alpha + beta and Liver Neoplasms
 Neoplasm Metastasis Antibodies  >2 publications with 14-3-3 alpha + beta and Neoplasm Metastasis
 Seizures Antibodies  >2 publications with 14-3-3 alpha + beta and Seizures

Organs/Tissues associated with anti-14-3-3 alpha + beta antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11 publications with 14-3-3 alpha + beta and Brain
 Blood Antibodies  >4 publications with 14-3-3 alpha + beta and Blood
 Muscle Antibodies  >4 publications with 14-3-3 alpha + beta and Muscle
 Kidney Antibodies  >3 publications with 14-3-3 alpha + beta and Kidney
 Liver Antibodies  >3 publications with 14-3-3 alpha + beta and Liver
 Nerve Antibodies  >3 publications with 14-3-3 alpha + beta and Nerve
 Lung Antibodies  >2 publications with 14-3-3 alpha + beta and Lung
 Ganglia Antibodies  >2 publications with 14-3-3 alpha + beta and Ganglia
 Vascular Antibodies  >2 publications with 14-3-3 alpha + beta and Vascular
 Heart Antibodies  >2 publications with 14-3-3 alpha + beta and Heart
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