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HSPB8 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179860

MBS179860 is an antibody targeting HSPB8. Also known as Anti-HSPB8/HSP22 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. Displays temperature-dependent chaperone activity. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HSPB8/HSP22, Monoclonal Antibody
Full Product Name
Anti-HSPB8/HSP22 Rabbit Monoclonal Antibody
Product Gene Name
anti-HSPB8 antibody
Product Synonym Names
Heat shock protein beta-8; HspB8; Alpha-crystallin C chain; E2-induced gene 1 protein; Protein kinase H11; Small stress protein-like protein HSP22; HSPB8; CRYAC; E2IG1; HSP22; PP1629
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
21604 MW
OMIM
AF191017 mRNA
3D Structure
ModBase 3D Structure for Q9UJY1
Immunogen
A synthesized peptide derived from human HSPB8/HSP22
Subcellular Localization
Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
Tissue Specificity
Predominantly expressed in skeletal muscle and heart.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-HSPB8 antibody

Signal Transduction; Protein Trafficking; chaperones; Heat Shock Proteins; Neuroscience; Neurology Process; neurodegenerative disease
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-HSPB8 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-HSPB8 antibody
WB: 1:500-1:2000
IHC: 1:50-1:100
ICC/IF: 1:50-1:100

Western Blot (WB) of anti-HSPB8 antibody

Western blot analysis of HSPB8/HSP22 expression in Human fetal heart lysate (MBS179860).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB8 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSPB8
Rabbit Anti-HSPB8 Monoclonal Antibody – Cat# MBS179860

Other Notes

Small volumes of anti-HSPB8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HSPB8. It may not necessarily be applicable to this product.

NCBI GI #
7657146
NCBI GeneID
26353
NCBI Accession #
NP_055180.1
UniProt Primary Accession #
Q9UJY1
UniProt Secondary Accession #
Q6FIH3; Q9UKS3; B2R6A6
UniProt Related Accession #
Q9UJY1
GenBank Nucleotide #
NM_014365.2
NCBI Official Full Name
heat shock protein beta-8
NCBI Official Synonym Full Names
heat shock protein family B (small) member 8
NCBI Official Symbol
HSPB8
NCBI Official Synonym Symbols
H11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22
UniProt Protein Name
Heat shock protein beta-8
UniProt Synonym Protein Names
Alpha-crystallin C chain; E2-induced gene 1 protein; Protein kinase H11; Small stress protein-like protein HSP22
Protein Family
Heat shock protein
UniProt Gene Name
HSPB8
UniProt Synonym Gene Names
CRYAC; E2IG1; HSP22; HspB8
NCBI Protein Information
heat shock protein beta-8

NCBI Summary for HSPB8

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008]
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UniProt Comments for HSPB8

Displays temperature-dependent chaperone activity.

Research Articles on HSPB8

1. suggest that HSPB8 may act as an intracellular factor against hepatitis C virus replication and that DNAJC5B has the same function, with more relevant results for genotype 3

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HSPB8 antibody

 Reference Product  PubMed Publications
 HSPB1 antibody  >56 publications with HSPB8 and HSPB1
 BAG3 antibody  >18 publications with HSPB8 and BAG3
 HSPB2 antibody  >12 publications with HSPB8 and HSPB2
 HSPB3 antibody  >10 publications with HSPB8 and HSPB3
 HSPB7 antibody  >7 publications with HSPB8 and HSPB7
 TRPV4 antibody  >5 publications with HSPB8 and TRPV4
 MPZ antibody  >5 publications with HSPB8 and MPZ
 CRYAB antibody  >5 publications with HSPB8 and CRYAB

Diseases associated with anti-HSPB8 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >50 publications with HSPB8 and Nervous System Diseases
 Congenital Abnormalities Antibodies  >25 publications with HSPB8 and Congenital Abnormalities
 Cardiovascular Diseases Antibodies  >18 publications with HSPB8 and Cardiovascular Diseases
 Heart Diseases Antibodies  >15 publications with HSPB8 and Heart Diseases
 Brain Diseases Antibodies  >11 publications with HSPB8 and Brain Diseases
 Hypertrophy Antibodies  >10 publications with HSPB8 and Hypertrophy
 Disease Models, Animal Antibodies  >7 publications with HSPB8 and Disease Models, Animal
 Breast Neoplasms Antibodies  >7 publications with HSPB8 and Breast Neoplasms
 Inflammation Antibodies  >5 publications with HSPB8 and Inflammation
 Neoplasms, Experimental Antibodies  >4 publications with HSPB8 and Neoplasms, Experimental

Organs/Tissues associated with anti-HSPB8 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >49 publications with HSPB8 and Muscle
 Heart Antibodies  >24 publications with HSPB8 and Heart
 Brain Antibodies  >22 publications with HSPB8 and Brain
 Nerve Antibodies  >20 publications with HSPB8 and Nerve
 Kidney Antibodies  >5 publications with HSPB8 and Kidney
 Skin Antibodies  >5 publications with HSPB8 and Skin
 Prostate Antibodies  >4 publications with HSPB8 and Prostate
 Vascular Antibodies  >3 publications with HSPB8 and Vascular
 Spinal Cord Antibodies  >3 publications with HSPB8 and Spinal Cord
 Bone Antibodies  >3 publications with HSPB8 and Bone
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