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ASPP2 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179869

Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ASPP2, Monoclonal Antibody
Full Product Name
Anti-ASPP2 Rabbit Monoclonal Antibody
Product Gene Name
anti-ASPP2 antibody
Product Synonym Gene Name
TP53BP2
Product Synonym Names
Apoptosis-stimulating of p53 protein 2; Bcl2-binding protein; Bbp; Renal carcinoma antigen NY-REN-51; Tumor suppressor p53-binding protein 2; 53BP2; p53-binding protein 2; p53BP2; TP53BP2; ASPP2; BBP
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
125616 MW
OMIM
U58334 mRNA
3D Structure
ModBase 3D Structure for Q13625
Immunogen
A synthesized peptide derived from human ASPP2
Subcellular Localization
Cytoplasm, perinuclear region. Nucleus. Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
Tissue Specificity
Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-ASPP2 antibody

Cell Biology; Cell Cycle; cell cycle Inhibitors; p53
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ASPP2 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-ASPP2 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200

Western Blot (WB) of anti-ASPP2 antibody

Western blot analysis of ASPP2 expression in MCF7 cell lysate (MBS179869).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53BP2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TP53BP2
Rabbit Anti-ASPP2 Monoclonal Antibody – Cat# MBS179869

Other Notes

Small volumes of anti-ASPP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ASPP2 purchase

MBS179869 is an antibody targeting ASPP2. Also known as Anti-ASPP2 Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ASPP2. It may not necessarily be applicable to this product.

NCBI GI #
112799849
NCBI GeneID
7159
NCBI Accession #
NP_001026855.2
UniProt Primary Accession #
Q13625
UniProt Secondary Accession #
Q12892; Q86X75; Q96KQ3; B4DG66
UniProt Related Accession #
Q13625
GenBank Nucleotide #
NM_001031685.2
NCBI Official Full Name
apoptosis-stimulating of p53 protein 2 isoform 1
NCBI Official Synonym Full Names
tumor protein p53 binding protein 2
NCBI Official Symbol
TP53BP2
NCBI Official Synonym Symbols
BBP; 53BP2; ASPP2; P53BP2; PPP1R13A
UniProt Protein Name
Apoptosis-stimulating of p53 protein 2
UniProt Synonym Protein Names
Bcl2-binding protein; Bbp; Renal carcinoma antigen NY-REN-51; Tumor suppressor p53-binding protein 2; 53BP2; p53-binding protein 2; p53BP2
UniProt Gene Name
TP53BP2
UniProt Synonym Gene Names
ASPP2; BBP; Bbp; 53BP2; p53-binding protein 2; p53BP2
NCBI Protein Information
apoptosis-stimulating of p53 protein 2

NCBI Summary for ASPP2

This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. It is localized to the perinuclear region of the cytoplasm, and regulates apoptosis and cell growth through interactions with other regulatory molecules including members of the p53 family. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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Research Articles on ASPP2

1. These findings identify TP53BP2 as a strong candidate causative gene for central nervous system (CNS) defects in 1q41q42 microdeletion syndrome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ASPP2 antibody

 Reference Product  PubMed Publications
 TP53 antibody  >12 publications with ASPP2 and TP53
 BCL2 antibody  >11 publications with ASPP2 and BCL2
 BAX antibody  >10 publications with ASPP2 and BAX

Pathways associated with anti-ASPP2 antibody

 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram

Diseases associated with anti-ASPP2 antibody

 Disease Name  Pubmed Publications
 Death Antibodies  >32 publications with ASPP2 and Death
 Lung Neoplasms Antibodies  >8 publications with ASPP2 and Lung Neoplasms
 Respiratory Tract Diseases Antibodies  >8 publications with ASPP2 and Respiratory Tract Diseases
 Lung Diseases Antibodies  >7 publications with ASPP2 and Lung Diseases
 Necrosis Antibodies  >7 publications with ASPP2 and Necrosis
 Inflammation Antibodies  >5 publications with ASPP2 and Inflammation
 Nerve Degeneration Antibodies  >3 publications with ASPP2 and Nerve Degeneration
 Liver Diseases Antibodies  >3 publications with ASPP2 and Liver Diseases
 Cell Transformation, Neoplastic Antibodies  >3 publications with ASPP2 and Cell Transformation, Neoplastic
 Kidney Diseases Antibodies  >1 publications with ASPP2 and Kidney Diseases

Organs/Tissues associated with anti-ASPP2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >13 publications with ASPP2 and Brain
 Lung Antibodies  >9 publications with ASPP2 and Lung
 Kidney Antibodies  >6 publications with ASPP2 and Kidney
 Stomach Antibodies  >5 publications with ASPP2 and Stomach
 Lymph Node Antibodies  >5 publications with ASPP2 and Lymph Node
 Liver Antibodies  >5 publications with ASPP2 and Liver
 Prostate Antibodies  >5 publications with ASPP2 and Prostate
 Blood Antibodies  >5 publications with ASPP2 and Blood
 Bone Antibodies  >2 publications with ASPP2 and Bone
 Heart Antibodies  >2 publications with ASPP2 and Heart
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