Rabbit Anti-HNRNPC Monoclonal Antibody – Cat# MBS179886

Western blot analysis of hnRNP C1/C2 expression in (1) HeLa cell lysate; (2) HepG2 cell lysate (MBS179886).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNRNPC monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HNRNPC

Hnrnp C1/C2 (HNRNPC) Antibody – Rabbit Monoclonal

Name: HNRNPC Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179886
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179886

Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed: 8264621). Specifically recognizes and binds N6- methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

hnRNP C1/C2 (HNRNPC), Monoclonal Antibody

Full Product Name

Anti-hnRNP C1/C2 Rabbit Monoclonal Antibody

Product Gene Name

anti-HNRNPC antibody

Product Synonym Names

Heterogeneous nuclear ribonucleoproteins C1/C2; hnRNP C1/C2; HNRNPC; HNRPC

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

33670 MW

OMIM

M29063 mRNA

3D Structure

ModBase 3D Structure for P07910

Immunogen

A synthesized peptide derived from human hnRNP C1/C2

Subcellular Localization

Nucleus. Component of ribonucleosomes.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-HNRNPC antibody

Epigenetics and Nuclear Signaling; Chromosome Structure; chromatin assembly

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-HNRNPC antibody

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-HNRNPC antibody

WB: 1:5000-1:20000
IHC: 1:100-1:200
ICC/IF: 1:100-1:200
IP: 1:50
FC/FACS: 1:50

Other Notes

Small volumes of anti-HNRNPC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HNRNPC purchase

MBS179886 is an antibody targeting HNRNPC. Also known as Anti-hnRNP C1/C2 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HNRNPC. It may not necessarily be applicable to this product.

NCBI GI #

117190192

NCBI GeneID

3183

NCBI Accession #

NP_001070910.1

UniProt Primary Accession #

P07910

UniProt Secondary Accession #

P22628; Q53EX2; Q59FD3; Q5FWE8; Q86SF8; Q86U45; Q96HK7; Q96HM4; Q96IY5; D3DS19; D3DS22

UniProt Related Accession #

P07910

GenBank Nucleotide #

NM_001077442.1

NCBI Official Full Name

heterogeneous nuclear ribonucleoproteins C1/C2 isoform a

NCBI Official Synonym Full Names

heterogeneous nuclear ribonucleoprotein C (C1/C2)

NCBI Official Symbol

HNRNPC

NCBI Official Synonym Symbols

C1; C2; HNRNP; HNRPC; SNRPC

UniProt Protein Name

Heterogeneous nuclear ribonucleoproteins C1/C2

Protein Family

Heterogeneous nuclear ribonucleoprotein

UniProt Gene Name

HNRNPC

UniProt Synonym Gene Names

HNRPC; hnRNP C1/C2

NCBI Protein Information

heterogeneous nuclear ribonucleoproteins C1/C2

NCBI Summary for HNRNPC

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for HNRNPC

Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed:8264621). May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm6A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671).

Research Articles on HNRNPC

1. APP levels then decrease progressively as a function of age in close relationship with the gradual normalization of FMRP and hnRNP C levels.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-HNRNPC antibody

Products by Pathway	Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies	Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
Regulation Of Telomerase Pathway antibodies	Regulation Of Telomerase Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
MRNA Splicing Pathway antibodies	MRNA Splicing Pathway Diagram
MRNA Splicing - Major Pathway antibodies	MRNA Splicing - Major Pathway Diagram
MRNA Processing Pathway antibodies	MRNA Processing Pathway Diagram

Diseases associated with anti-HNRNPC antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>7 publications with HNRNPC and Neoplasms
Carcinoma Antibodies	>3 publications with HNRNPC and Carcinoma
Lung Diseases Antibodies	>3 publications with HNRNPC and Lung Diseases
Brain Diseases Antibodies	>2 publications with HNRNPC and Brain Diseases
Breast Neoplasms Antibodies	>2 publications with HNRNPC and Breast Neoplasms
Death Antibodies	>2 publications with HNRNPC and Death
Inflammation Antibodies	>2 publications with HNRNPC and Inflammation
Neoplasm Metastasis Antibodies	>1 publications with HNRNPC and Neoplasm Metastasis
Carcinoma, Hepatocellular Antibodies	>1 publications with HNRNPC and Carcinoma, Hepatocellular
Cardiovascular Diseases Antibodies	>1 publications with HNRNPC and Cardiovascular Diseases

Organs/Tissues associated with anti-HNRNPC antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>7 publications with HNRNPC and Lung
Brain Antibodies	>4 publications with HNRNPC and Brain
Kidney Antibodies	>1 publications with HNRNPC and Kidney