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TIA1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179887

Anti-TIA1 Rabbit Monoclonal Antibody tested for IP, IF, IHC, ICC, WB in Human, Mouse More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TIA1, Monoclonal Antibody
Full Product Name
Anti-TIA1 Rabbit Monoclonal Antibody
Product Gene Name
anti-TIA1 antibody
Product Synonym Names
Nucleolysin TIA-1 isoform p40; RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA-1; p40-TIA-1; TIA1
Clonality
Monoclonal
Isotype
IgG
Clone Number
AAHD-20
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-chromatography
Concentration
0.5 - 1mg/ml (lot specific)
Preparation and Storage
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
OMIM
M77142 mRNA
3D Structure
ModBase 3D Structure for P31483
Immunogen
A synthesized peptide derived from human TIA1

Product Categories/Family for anti-TIA1 antibody

Cell Biology; Apoptosis; Nucleus; Immunology; Adaptive Immunity; T Cells; NON-CD; Epigenetics and Nuclear Signaling; DNA/RNA; RNA Processing; Cancer; Cell Death
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TIA1 antibody
Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-TIA1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50

Western Blot (WB) of anti-TIA1 antibody

Western blot analysis of TIA1 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate (MBS179887).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIA1 monoclonal antibody overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TIA1
Rabbit Anti-TIA1 Monoclonal Antibody – Cat# MBS179887

Other Notes

Small volumes of anti-TIA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIA1 purchase

MBS179887 is an antibody targeting TIA1. Also known as Anti-TIA1 Rabbit Monoclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TIA1. It may not necessarily be applicable to this product.

NCBI GI #
188219593
NCBI GeneID
7072
NCBI Accession #
NP_071320.2
UniProt Primary Accession #
P31483
UniProt Secondary Accession #
Q53SS9; Q96B58
UniProt Related Accession #
P31483
GenBank Nucleotide #
NM_022037.3
NCBI Official Full Name
nucleolysin TIA-1 isoform p40 isoform 1
NCBI Official Synonym Full Names
TIA1 cytotoxic granule associated RNA binding protein
NCBI Official Symbol
TIA1
NCBI Official Synonym Symbols
WDM; TIA-1
UniProt Protein Name
Nucleolysin TIA-1 isoform p40
UniProt Synonym Protein Names
RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA-1; p40-TIA-1
Protein Family
Nucleolysin
UniProt Gene Name
TIA1
UniProt Synonym Gene Names
TIA-1
NCBI Protein Information
nucleolysin TIA-1 isoform p40; nucleolysin TIA-1

NCBI Summary for TIA1

The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms has been found for this gene. [provided by RefSeq, May 2017]
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UniProt Comments for TIA1

Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.

Research Articles on TIA1

1. Downregulation of TIA-1-enhanced mitochondrial elongation, whereas ectopic expression of TIA-1 resulted in mitochondria fragmentation. In addition, TIA-1 increased mitochondrial activity, including the rate of ATP synthesis and oxygen consumption.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TIA1 antibody

 Reference Product  PubMed Publications
 GZMB antibody  >64 publications with TIA1 and GZMB
 PTGS2 antibody  >5 publications with TIA1 and PTGS2
 G3BP1 antibody  >4 publications with TIA1 and G3BP1
 DCP1A antibody  >3 publications with TIA1 and DCP1A
 IL10 antibody  >3 publications with TIA1 and IL10
 FASTK antibody  >3 publications with TIA1 and FASTK
 COL2A1 antibody  >2 publications with TIA1 and COL2A1

Diseases associated with anti-TIA1 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >33 publications with TIA1 and Necrosis
 Skin Neoplasms Antibodies  >28 publications with TIA1 and Skin Neoplasms
 Inflammation Antibodies  >22 publications with TIA1 and Inflammation
 Nervous System Diseases Antibodies  >21 publications with TIA1 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >20 publications with TIA1 and Neoplasms, Experimental
 Liver Diseases Antibodies  >17 publications with TIA1 and Liver Diseases
 Liver Neoplasms Antibodies  >11 publications with TIA1 and Liver Neoplasms
 Brain Diseases Antibodies  >10 publications with TIA1 and Brain Diseases
 Fibrosis Antibodies  >7 publications with TIA1 and Fibrosis
 Anemia Antibodies  >7 publications with TIA1 and Anemia

Organs/Tissues associated with anti-TIA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >183 publications with TIA1 and Blood
 Skin Antibodies  >54 publications with TIA1 and Skin
 Bone Antibodies  >36 publications with TIA1 and Bone
 Bone Marrow Antibodies  >34 publications with TIA1 and Bone Marrow
 Lymph Node Antibodies  >26 publications with TIA1 and Lymph Node
 Liver Antibodies  >26 publications with TIA1 and Liver
 Spleen Antibodies  >18 publications with TIA1 and Spleen
 Kidney Antibodies  >15 publications with TIA1 and Kidney
 Brain Antibodies  >15 publications with TIA1 and Brain
 Lung Antibodies  >14 publications with TIA1 and Lung
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