Rabbit Anti-UBA1 Monoclonal Antibody – Cat# MBS179897

Western blot analysis of UBA1 expression in Jurkat cell lysate (MBS179897).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for UBA1

UBA1 Antibody – Rabbit Monoclonal

Name: UBA1 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179897
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179897

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

UBA1/Ube1, Monoclonal Antibody

Full Product Name

Anti-UBA1/Ube1 Rabbit Monoclonal Antibody

Product Gene Name

anti-UBA1 antibody

Product Synonym Names

Ubiquitin-like modifier-activating enzyme 1; 6.2.1.45; Protein A1S9; Ubiquitin-activating enzyme E1; UBA1; A1S9T; UBE1

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

117849 MW

OMIM

X56976 mRNA

3D Structure

ModBase 3D Structure for P22314

Immunogen

A synthesized peptide derived from human UBA1

Subcellular Localization

Cytoplasm. Mitochondrion. Nucleus.

Tissue Specificity

Detected in erythrocytes (at protein level). Ubiquitous.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-UBA1 antibody

Cell Biology; Proteolysis/Ubiquitin; Proteasome/Ubiquitin; Epigenetics and Nuclear Signaling; ubiquitin & ubiquitin like modifiers; e1 ubiquitin activating Enzymes

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-UBA1 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-UBA1 antibody

WB: 1:1000-1:2000
IHC: 1:50-1:100
ICC/IF: 1:50-1:200
FC/FACS: 1:20

Other Notes

Small volumes of anti-UBA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBA1 purchase

MBS179897 is an antibody targeting UBA1. Also known as Anti-UBA1/Ube1 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for UBA1. It may not necessarily be applicable to this product.

NCBI GI #

23510338

NCBI GeneID

7317

NCBI Accession #

NP_003325.2

UniProt Primary Accession #

P22314

UniProt Secondary Accession #

Q5JRR8; Q96E13

UniProt Related Accession #

P22314

GenBank Nucleotide #

NM_003334.3

NCBI Official Full Name

ubiquitin-like modifier-activating enzyme 1

NCBI Official Synonym Full Names

ubiquitin like modifier activating enzyme 1

NCBI Official Symbol

UBA1

NCBI Official Synonym Symbols

A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; CFAP124

UniProt Protein Name

Ubiquitin-like modifier-activating enzyme 1

UniProt Synonym Protein Names

Protein A1S9; Ubiquitin-activating enzyme E1

Protein Family

Ubiquitin-activating enzyme

UniProt Gene Name

UBA1

UniProt Synonym Gene Names

A1S9T; UBE1

NCBI Protein Information

ubiquitin-like modifier-activating enzyme 1

NCBI Summary for UBA1

The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]

UniProt Comments for UBA1

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).

Research Articles on UBA1

1. recent findings put UBA1 at the center of cellular homeostasis and neurodegeneration [review]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UBA1 antibody

Reference Product	PubMed Publications
UBC antibody	>2 publications with UBA1 and UBC
UBE2A antibody	>1 publications with UBA1 and UBE2A

Pathways associated with anti-UBA1 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies	Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies	Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Parkin-Ubiquitin Proteasomal System Pathway antibodies	Parkin-Ubiquitin Proteasomal System Pathway Diagram
Parkinson's Disease Pathway antibodies	Parkinson's Disease Pathway Diagram
Parkinsons Disease Pathway antibodies	Parkinsons Disease Pathway Diagram
Proteasome Degradation Pathway antibodies	Proteasome Degradation Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with anti-UBA1 antibody

Disease Name	Pubmed Publications
Atrophy Antibodies	>10 publications with UBA1 and Atrophy
Death Antibodies	>9 publications with UBA1 and Death
Nervous System Diseases Antibodies	>8 publications with UBA1 and Nervous System Diseases
Cardiovascular Diseases Antibodies	>3 publications with UBA1 and Cardiovascular Diseases
Weight Loss Antibodies	>3 publications with UBA1 and Weight Loss
Brain Diseases Antibodies	>3 publications with UBA1 and Brain Diseases
Necrosis Antibodies	>3 publications with UBA1 and Necrosis
Inflammation Antibodies	>2 publications with UBA1 and Inflammation
Adenocarcinoma Antibodies	>2 publications with UBA1 and Adenocarcinoma
Heart Diseases Antibodies	>2 publications with UBA1 and Heart Diseases

Organs/Tissues associated with anti-UBA1 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>7 publications with UBA1 and Muscle
Brain Antibodies	>7 publications with UBA1 and Brain
Nerve Antibodies	>4 publications with UBA1 and Nerve
Eye Antibodies	>3 publications with UBA1 and Eye
Spinal Cord Antibodies	>3 publications with UBA1 and Spinal Cord
Blood Antibodies	>3 publications with UBA1 and Blood
Intestine Antibodies	>2 publications with UBA1 and Intestine
Lung Antibodies	>2 publications with UBA1 and Lung
Embryonic Tissue Antibodies	>2 publications with UBA1 and Embryonic Tissue
Mouth Antibodies	>2 publications with UBA1 and Mouth