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ILK Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179915

Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of… More details

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Product Overview

Online Datasheet in PDF
Product Name
ILK/Integrin Linked Ilk, Monoclonal Antibody
Full Product Name
Anti-ILK/Integrin Linked Ilk Rabbit Monoclonal Antibody
Product Gene Name
anti-ILK antibody
Product Synonym Names
Integrin-linked protein kinase; 2.7.11.1; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK; ILK; ILK1; ILK2
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
51419 MW
OMIM
U40282 mRNA
3D Structure
ModBase 3D Structure for Q13418
Immunogen
A synthesized peptide derived from human ILK
Subcellular Localization
Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium. Cytoplasm, myofibril, sarcomere.
Tissue Specificity
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-ILK antibody

Signal Transduction; Protein Phosphorylation; tyrosine Kinases
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ILK antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-ILK antibody
WB: 1:1000-1:5000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50

Western Blot (WB) of anti-ILK antibody

Western blot analysis of ILK expression in K562 cell lysate (MBS179915).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILK monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ILK
Rabbit Anti-ILK Monoclonal Antibody – Cat# MBS179915

Other Notes

Small volumes of anti-ILK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ILK purchase

MBS179915 is an antibody targeting ILK. Also known as Anti-ILK/Integrin Linked Ilk Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ILK. It may not necessarily be applicable to this product.

NCBI GI #
62420873
NCBI GeneID
3611
NCBI Accession #
NP_001014794.1
UniProt Primary Accession #
Q13418
UniProt Secondary Accession #
P57043; Q68DZ3; B7Z1I0; B7Z418; D3DQU0
UniProt Related Accession #
Q13418
GenBank Nucleotide #
NM_001014794.2
NCBI Official Full Name
integrin-linked protein kinase isoform 1
NCBI Official Synonym Full Names
integrin linked kinase
NCBI Official Symbol
ILK
NCBI Official Synonym Symbols
P59; ILK-1; ILK-2; p59ILK; HEL-S-28
UniProt Protein Name
Integrin-linked protein kinase
UniProt Synonym Protein Names
59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK
Protein Family
Integrin-linked protein kinase
UniProt Gene Name
ILK
UniProt Synonym Gene Names
ILK1; ILK2
NCBI Protein Information
integrin-linked protein kinase

NCBI Summary for ILK

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
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UniProt Comments for ILK

Receptor-proximal protein kinase regulating integrin-mediated signal transduction (PubMed:8538749, PubMed:9736715). May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (PubMed:8538749, PubMed:9736715).
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Research Articles on ILK

1. The current data support MT1-MMP as an additional ILK substrate and show that modulation of ILK expression and activity inhibit MT1-MMP-related pro-metastatic behaviors of ovarian cancer cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ILK antibody

 Reference Product  PubMed Publications
 LIMS1 antibody  >41 publications with ILK and LIMS1
 PARVA antibody  >36 publications with ILK and PARVA
 AKT1 antibody  >36 publications with ILK and AKT1
 PARVB antibody  >22 publications with ILK and PARVB
 PXN antibody  >9 publications with ILK and PXN
 CASP9 antibody  >5 publications with ILK and CASP9
 ILKAP antibody  >4 publications with ILK and ILKAP
 ITGB2 antibody  >3 publications with ILK and ITGB2
 CAV1 antibody  >2 publications with ILK and CAV1
 PARVG antibody  >2 publications with ILK and PARVG

Pathways associated with anti-ILK antibody

 Products by Pathway  Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Cell-extracellular Matrix Interactions Pathway antibodies  Cell-extracellular Matrix Interactions Pathway Diagram
 Endometrial Cancer Pathway antibodies  Endometrial Cancer Pathway Diagram
 Endometrial Cancer Pathway antibodies  Endometrial Cancer Pathway Diagram
 Eukaryotic Transcription Initiation Pathway antibodies  Eukaryotic Transcription Initiation Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram

Diseases associated with anti-ILK antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >206 publications with ILK and Neoplasms
 Fibrosis Antibodies  >38 publications with ILK and Fibrosis
 Kidney Diseases Antibodies  >35 publications with ILK and Kidney Diseases
 Breast Neoplasms Antibodies  >29 publications with ILK and Breast Neoplasms
 Heart Diseases Antibodies  >27 publications with ILK and Heart Diseases
 Inflammation Antibodies  >25 publications with ILK and Inflammation
 Liver Diseases Antibodies  >24 publications with ILK and Liver Diseases
 Lung Diseases Antibodies  >23 publications with ILK and Lung Diseases
 Lung Neoplasms Antibodies  >22 publications with ILK and Lung Neoplasms
 Brain Diseases Antibodies  >17 publications with ILK and Brain Diseases

Organs/Tissues associated with anti-ILK antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >118 publications with ILK and Muscle
 Vascular Antibodies  >88 publications with ILK and Vascular
 Blood Antibodies  >85 publications with ILK and Blood
 Kidney Antibodies  >79 publications with ILK and Kidney
 Embryonic Tissue Antibodies  >51 publications with ILK and Embryonic Tissue
 Heart Antibodies  >47 publications with ILK and Heart
 Bone Antibodies  >39 publications with ILK and Bone
 Lung Antibodies  >38 publications with ILK and Lung
 Liver Antibodies  >34 publications with ILK and Liver
 Brain Antibodies  >31 publications with ILK and Brain
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