Rabbit Anti-EGLN2 Monoclonal Antibody – Cat# MBS179925

Western blot analysis of PHD1 in (1) HeLa cell lysate; (2) A549 cell lysate (MBS179925).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGLN2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EGLN2

PHD1/Prolyl Hydroxylase (EGLN2) Antibody – Rabbit Monoclonal

Name: EGLN2 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179925
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179925

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD)… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PHD1/prolyl hydroxylase (EGLN2), Monoclonal Antibody

Full Product Name

Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody

Product Gene Name

anti-EGLN2 antibody

Product Synonym Names

Egl nine homolog 2; 1.14.11.29; Estrogen-induced tag 6; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; Prolyl hydroxylase domain-containing protein 1; PHD1; EGLN2; EIT6

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

43650 MW

OMIM

AJ310544 mRNA

3D Structure

ModBase 3D Structure for Q96KS0

Immunogen

A synthesized peptide derived from human PHD1/prolyl hydroxylase

Subcellular Localization

Nucleus.

Tissue Specificity

Expressed in adult and fetal heart, brain, liver, lung, skeletal muscle, and kidney. Also expressed in testis and placenta. Highest levels in adult brain, placenta, lung, kidney, and testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-EGLN2 antibody

Cardiovascular; Hypoxia; Prolyl Hydroxylase; Epigenetics and Nuclear Signaling; Cardiovascular/Immune; Cancer; Invasion/Microenvironment; Hydroxylases; Cancer Metabolism; Response to Hypoxia; Metabolism; Pathways and Processes; Metabolism Processes; Types

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-EGLN2 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-EGLN2 antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Other Notes

Small volumes of anti-EGLN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EGLN2 purchase

MBS179925 is an antibody targeting EGLN2. Also known as Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EGLN2. It may not necessarily be applicable to this product.

NCBI GI #

16604260

NCBI GeneID

112398

NCBI Accession #

NP_444274.1

UniProt Primary Accession #

Q96KS0

UniProt Secondary Accession #

Q8WWY4; Q9BV14; A8K5S0

UniProt Related Accession #

Q96KS0

GenBank Nucleotide #

NM_053046.3

NCBI Official Full Name

egl nine homolog 2

NCBI Official Synonym Full Names

egl-9 family hypoxia inducible factor 2

NCBI Official Symbol

EGLN2

NCBI Official Synonym Symbols

EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1

UniProt Protein Name

Egl nine homolog 2

UniProt Synonym Protein Names

Estrogen-induced tag 6; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; Prolyl hydroxylase domain-containing protein 1; PHD1

Protein Family

Egl nine

UniProt Gene Name

EGLN2

UniProt Synonym Gene Names

EIT6; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; PHD1

NCBI Protein Information

egl nine homolog 2

NCBI Summary for EGLN2

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]

Research Articles on EGLN2

1. In advanced-stage Hodgkin's Lymphoma patients strong cytoplasmic PHD1 expression in Reed-Sternberg cells was associated with poor relapse-free survival among patients treated with involved-field radiotherapy and advanced-stage patients treated with doxorubicin, bleomycin, vinblastine and darcabazine and involved-field radiotherapy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EGLN2 antibody

Reference Product	PubMed Publications
HIF1A antibody	>12 publications with EGLN2 and HIF1A

Pathways associated with anti-EGLN2 antibody

Products by Pathway	Pathway Diagram
HIF-2-alpha Transcription Factor Network Pathway antibodies	HIF-2-alpha Transcription Factor Network Pathway Diagram
Pathways In Cancer antibodies	Pathways In Cancer Diagram
Renal Cell Carcinoma Pathway antibodies	Renal Cell Carcinoma Pathway Diagram
Renal Cell Carcinoma Pathway antibodies	Renal Cell Carcinoma Pathway Diagram

Diseases associated with anti-EGLN2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>12 publications with EGLN2 and Neoplasms
Carcinoma Antibodies	>8 publications with EGLN2 and Carcinoma
Adenocarcinoma Antibodies	>4 publications with EGLN2 and Adenocarcinoma
Breast Neoplasms Antibodies	>4 publications with EGLN2 and Breast Neoplasms
Skin Diseases Antibodies	>4 publications with EGLN2 and Skin Diseases
Kidney Diseases Antibodies	>4 publications with EGLN2 and Kidney Diseases
Carcinoma, Renal Cell Antibodies	>3 publications with EGLN2 and Carcinoma, Renal Cell
Lung Neoplasms Antibodies	>2 publications with EGLN2 and Lung Neoplasms
Anemia Antibodies	>2 publications with EGLN2 and Anemia
Neoplasm Metastasis Antibodies	>1 publications with EGLN2 and Neoplasm Metastasis

Organs/Tissues associated with anti-EGLN2 antibody

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>4 publications with EGLN2 and Kidney
Vascular Antibodies	>3 publications with EGLN2 and Vascular
Muscle Antibodies	>3 publications with EGLN2 and Muscle
Blood Antibodies	>2 publications with EGLN2 and Blood
Lung Antibodies	>2 publications with EGLN2 and Lung
Intestine Antibodies	>1 publications with EGLN2 and Intestine
Mammary Gland Antibodies	>1 publications with EGLN2 and Mammary Gland
Embryonic Tissue Antibodies	>1 publications with EGLN2 and Embryonic Tissue
Placenta Antibodies	>1 publications with EGLN2 and Placenta
Adrenal Gland Antibodies	>1 publications with EGLN2 and Adrenal Gland