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HSP47 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179932

Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Hsp47, Monoclonal Antibody
Full Product Name
Anti-Hsp47 Rabbit Monoclonal Antibody
Product Gene Name
anti-Hsp47 antibody
Product Synonym Gene Name
SERPINH1
Product Synonym Names
Serpin H1; 47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP3; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; Colligin; Rheumatoid arthritis-related antigen RA-A47; SERPINH1; CBP1; CBP2; HSP47; SERPINH2; PIG14
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
46441 MW
OMIM
X61598 mRNA
3D Structure
ModBase 3D Structure for P50454
Immunogen
A synthesized peptide derived from human Hsp47
Subcellular Localization
Endoplasmic reticulum lumen.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-Hsp47 antibody

Signal Transduction; Protein Trafficking; chaperones; Heat Shock Proteins; Developmental Biology; Organogenesis; skeletal development; Bone; Kits/Lysates/Other; ELISA Kits; Developmental Proteins ELISA Kits; signal transduction proteins ELISA Kits; Protea
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Hsp47 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-Hsp47 antibody
WB: 1:500-1:1000
IHC: 1:50-1:200

Western Blot (WB) of anti-Hsp47 antibody

Western blot analysis of Hsp47 expression in HeLa cell lysate (MBS179932).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINH1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SERPINH1
Rabbit Anti-HSP47 Monoclonal Antibody – Cat# MBS179932

Other Notes

Small volumes of anti-Hsp47 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hsp47 purchase

MBS179932 is an antibody targeting Hsp47. Also known as Anti-Hsp47 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Hsp47. It may not necessarily be applicable to this product.

NCBI GI #
333360851
NCBI GeneID
871
NCBI Accession #
NP_001193943.1
UniProt Primary Accession #
P50454
UniProt Secondary Accession #
P29043; Q5XPB4; Q6NSJ6; Q8IY96; Q9NP88; B3KVJ3
UniProt Related Accession #
P50454
GenBank Nucleotide #
NM_001207014.1
NCBI Official Full Name
serpin H1
NCBI Official Synonym Full Names
serpin family H member 1
NCBI Official Symbol
SERPINH1
NCBI Official Synonym Symbols
CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
UniProt Protein Name
Serpin H1
UniProt Synonym Protein Names
47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP3; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; Colligin; Rheumatoid arthritis-related antigen RA-A47
UniProt Gene Name
SERPINH1
UniProt Synonym Gene Names
CBP1; CBP2; HSP47; SERPINH2; AsTP3; Colligin
NCBI Protein Information
serpin H1

NCBI Summary for Hsp47

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]
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Research Articles on Hsp47

1. miR-29b can reduce collagen biosynthesis during skin wound healing likely via post-transcriptional inhibition of HSP47 expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Hsp47 antibody

 Reference Product  PubMed Publications
 LEPRE1 antibody  >10 publications with Hsp47 and LEPRE1
 CRTAP antibody  >10 publications with Hsp47 and CRTAP
 PPIB antibody  >9 publications with Hsp47 and PPIB
 AKT1 antibody  >1 publications with Hsp47 and AKT1
 FN1 antibody  >1 publications with Hsp47 and FN1

Pathways associated with anti-Hsp47 antibody

 Products by Pathway  Pathway Diagram
 Collagen Biosynthesis And Modifying Enzymes Pathway antibodies  Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram

Diseases associated with anti-Hsp47 antibody

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >72 publications with Hsp47 and Fibrosis
 Kidney Diseases Antibodies  >28 publications with Hsp47 and Kidney Diseases
 Disease Models, Animal Antibodies  >26 publications with Hsp47 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >18 publications with Hsp47 and Cardiovascular Diseases
 Inflammation Antibodies  >15 publications with Hsp47 and Inflammation
 Nervous System Diseases Antibodies  >11 publications with Hsp47 and Nervous System Diseases
 Necrosis Antibodies  >10 publications with Hsp47 and Necrosis
 Liver Diseases Antibodies  >8 publications with Hsp47 and Liver Diseases
 Neoplasms, Experimental Antibodies  >7 publications with Hsp47 and Neoplasms, Experimental
 Liver Cirrhosis Antibodies  >7 publications with Hsp47 and Liver Cirrhosis

Organs/Tissues associated with anti-Hsp47 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >51 publications with Hsp47 and Muscle
 Kidney Antibodies  >37 publications with Hsp47 and Kidney
 Blood Antibodies  >28 publications with Hsp47 and Blood
 Bone Antibodies  >28 publications with Hsp47 and Bone
 Connective Tissue Antibodies  >28 publications with Hsp47 and Connective Tissue
 Vascular Antibodies  >26 publications with Hsp47 and Vascular
 Skin Antibodies  >23 publications with Hsp47 and Skin
 Lung Antibodies  >20 publications with Hsp47 and Lung
 Liver Antibodies  >15 publications with Hsp47 and Liver
 Embryonic Tissue Antibodies  >14 publications with Hsp47 and Embryonic Tissue
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