Rabbit Anti-NQO2 Monoclonal Antibody – Cat# MBS179937

Western blot analysis of NQO2 expression in Mouse heart lysate (MBS179937).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NQO2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NQO2

NQO2 Antibody – Rabbit Monoclonal

Name: NQO2 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179937
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179937

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NQO2, Monoclonal Antibody

Full Product Name

Anti-NQO2 Rabbit Monoclonal Antibody

Product Gene Name

anti-NQO2 antibody

Product Synonym Names

Ribosyldihydronicotinamide dehydrogenase [quinone]; 1.10.5.1; NRH dehydrogenase [quinone] 2; NRH: quinone oxidoreductase 2; Quinone reductase 2; QR2; NQO2; NMOR2

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

25919 MW

OMIM

J02888 mRNA

3D Structure

ModBase 3D Structure for P16083

Immunogen

A synthesized peptide derived from human NQO2

Subcellular Localization

Cytoplasm.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-NQO2 antibody

Signal Transduction; Metabolism; Energy Metabolism; Signal Transduction; Drug Metabolism; Cell Biology; Other Antibodies; drugs and chemicals; Metabolism; Pathways and Processes; Metabolic Signaling Pathways; energy transfer Pathways

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-NQO2 antibody

Flow Cytometry (FC/FACS), Western Blot (WB)

Application Notes for anti-NQO2 antibody

WB: 1:500-1:2000
FC/FACS: 1:50

Other Notes

Small volumes of anti-NQO2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NQO2 purchase

MBS179937 is an antibody targeting NQO2. Also known as Anti-NQO2 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NQO2. It may not necessarily be applicable to this product.

NCBI GI #

156564357

NCBI GeneID

4835

NCBI Accession #

NP_000895.2

UniProt Primary Accession #

P16083

UniProt Secondary Accession #

Q5TD04; B2R492

UniProt Related Accession #

P16083

GenBank Nucleotide #

NM_000904.4

NCBI Official Full Name

ribosyldihydronicotinamide dehydrogenase

NCBI Official Synonym Full Names

N-ribosyldihydronicotinamide:quinone reductase 2

NCBI Official Symbol

NQO2

NCBI Official Synonym Symbols

QR2; DHQV; DIA6; NMOR2

UniProt Protein Name

Ribosyldihydronicotinamide dehydrogenase [quinone]

UniProt Synonym Protein Names

NRH dehydrogenase [quinone] 2; NRH:quinone oxidoreductase 2; Quinone reductase 2; QR2

Protein Family

NADH-quinone oxidoreductase

UniProt Gene Name

NQO2

UniProt Synonym Gene Names

NMOR2; QR2

NCBI Protein Information

ribosyldihydronicotinamide dehydrogenase [quinone]

NCBI Summary for NQO2

This gene encodes a member of the thioredoxin family of enzymes. It is a cytosolic and ubiquitously expressed flavoprotein that catalyzes the two-electron reduction of quinone substrates and uses dihydronicotinamide riboside as a reducing coenzyme. Mutations in this gene have been associated with neurodegenerative diseases and several cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Research Articles on NQO2

1. The ontogeny and population variability of human hepatic dihydronicotinamide riboside:quinone oxidoreductase (NQO2).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NQO2 antibody

Reference Product	PubMed Publications
TP53 antibody	>2 publications with NQO2 and TP53

Diseases associated with anti-NQO2 antibody

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>10 publications with NQO2 and Breast Neoplasms
Neoplasms, Experimental Antibodies	>9 publications with NQO2 and Neoplasms, Experimental
Bone Marrow Diseases Antibodies	>8 publications with NQO2 and Bone Marrow Diseases
Nervous System Diseases Antibodies	>7 publications with NQO2 and Nervous System Diseases
Hyperplasia Antibodies	>6 publications with NQO2 and Hyperplasia
Liver Neoplasms Antibodies	>6 publications with NQO2 and Liver Neoplasms
Liver Diseases Antibodies	>5 publications with NQO2 and Liver Diseases
Necrosis Antibodies	>4 publications with NQO2 and Necrosis
Poisoning Antibodies	>4 publications with NQO2 and Poisoning
Skin Neoplasms Antibodies	>4 publications with NQO2 and Skin Neoplasms

Organs/Tissues associated with anti-NQO2 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>10 publications with NQO2 and Brain
Kidney Antibodies	>6 publications with NQO2 and Kidney
Spleen Antibodies	>3 publications with NQO2 and Spleen