Rabbit Anti-CLTC Monoclonal Antibody – Cat# MBS179940

Western blot analysis of Clathrin heavy chain expression in Hela cell lysate (MBS179940).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLTC monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CLTC

Clathrin Heavy Chain (CLTC) Antibody – Rabbit Monoclonal

Name: CLTC Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179940
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179940

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Clathrin heavy chain (CLTC), Monoclonal Antibody

Full Product Name

Anti-Clathrin heavy chain Rabbit Monoclonal Antibody

Product Gene Name

anti-CLTC antibody

Product Synonym Names

Clathrin heavy chain 1; Clathrin heavy chain on chromosome 17; CLH-17; CLTC; CLH17; CLTCL2; KIAA0034

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

191615 MW

OMIM

D21260 mRNA

3D Structure

ModBase 3D Structure for Q00610

Immunogen

A synthesized peptide derived from human Clathrin heavy chain

Subcellular Localization

Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CLTC antibody

Signal Transduction; Protein Trafficking; Vesicle Transport; coat proteins; Tags & Cell Markers; Subcellular Markers; organelles; caveolae and clathrin

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-CLTC antibody

Immunohistochemistry (IHC), Western Blot (WB)

Application Notes for anti-CLTC antibody

WB: 1:1000-1:5000
IHC: 1:50-1:200

Other Notes

Small volumes of anti-CLTC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CLTC purchase

MBS179940 is an antibody targeting CLTC. Also known as Anti-Clathrin heavy chain Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CLTC. It may not necessarily be applicable to this product.

NCBI GI #

4758012

NCBI GeneID

1213

NCBI Accession #

NP_004850.1

UniProt Primary Accession #

Q00610

UniProt Secondary Accession #

Q6N0A0; Q86TF2; D3DU00

UniProt Related Accession #

Q00610

GenBank Nucleotide #

NM_004859.3

NCBI Official Full Name

clathrin heavy chain 1 isoform 1

NCBI Official Synonym Full Names

clathrin heavy chain

NCBI Official Symbol

CLTC

NCBI Official Synonym Symbols

Hc; CHC; CHC17; CLH-17; CLTCL2

UniProt Protein Name

Clathrin heavy chain 1

UniProt Synonym Protein Names

Clathrin heavy chain on chromosome 17; CLH-17

Protein Family

Clathrin

UniProt Gene Name

CLTC

UniProt Synonym Gene Names

CLH17; CLTCL2; KIAA0034; CLH-17

NCBI Protein Information

clathrin heavy chain 1

NCBI Summary for CLTC

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]

UniProt Comments for CLTC

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Plays a role in early autophagosome formation (PubMed:20639872).

Research Articles on CLTC

1. this study shows that clathrin, including the clathrin population associated to internal vesicles, controls lymphocyte migration by regulating actin rearrangements occurring at the leading age

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CLTC antibody

Reference Product	PubMed Publications
CLTA antibody	>3 publications with CLTC and CLTA
AP2A1 antibody	>1 publications with CLTC and AP2A1

Pathways associated with anti-CLTC antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Arf6 Trafficking Events Pathway antibodies	Arf6 Trafficking Events Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Clathrin Derived Vesicle Budding Pathway antibodies	Clathrin Derived Vesicle Budding Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
EPH-Ephrin Signaling Pathway antibodies	EPH-Ephrin Signaling Pathway Diagram
EPH-ephrin Mediated Repulsion Of Cells Pathway antibodies	EPH-ephrin Mediated Repulsion Of Cells Pathway Diagram
Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies	Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram

Diseases associated with anti-CLTC antibody

Disease Name	Pubmed Publications
Chromosome Aberrations Antibodies	>14 publications with CLTC and Chromosome Aberrations
Lymphoma, Large B-Cell, Diffuse Antibodies	>12 publications with CLTC and Lymphoma, Large B-Cell, Diffuse
Carcinoma Antibodies	>9 publications with CLTC and Carcinoma
Adenocarcinoma Antibodies	>6 publications with CLTC and Adenocarcinoma
Kidney Diseases Antibodies	>6 publications with CLTC and Kidney Diseases
Inflammation Antibodies	>3 publications with CLTC and Inflammation
Lung Neoplasms Antibodies	>2 publications with CLTC and Lung Neoplasms
Disease Models, Animal Antibodies	>1 publications with CLTC and Disease Models, Animal
Anemia Antibodies	>1 publications with CLTC and Anemia
Breast Neoplasms Antibodies	>1 publications with CLTC and Breast Neoplasms

Organs/Tissues associated with anti-CLTC antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>12 publications with CLTC and Muscle
Kidney Antibodies	>7 publications with CLTC and Kidney
Blood Antibodies	>6 publications with CLTC and Blood
Lymph Antibodies	>5 publications with CLTC and Lymph
Lymph Node Antibodies	>5 publications with CLTC and Lymph Node
Lung Antibodies	>3 publications with CLTC and Lung
Bone Marrow Antibodies	>3 publications with CLTC and Bone Marrow
Bone Antibodies	>3 publications with CLTC and Bone
Liver Antibodies	>2 publications with CLTC and Liver
Stomach Antibodies	>2 publications with CLTC and Stomach