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SHIP Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179954

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol- 3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SHIP, Monoclonal Antibody
Full Product Name
Anti-SHIP Rabbit Monoclonal Antibody
Product Gene Name
anti-SHIP antibody
Product Synonym Gene Name
INPP5D
Product Synonym Names
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; 3.1.3.86; Inositol polyphosphate-5-phosphatase of 145 kDa; SIP-145; SH2 domain-containing inositol 5'-phosphatase 1; SH2 domain-containing inositol phosphatase 1; SHIP-1; p150Ship; hp51CN; INPP5D; SHIP; SHIP1
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
133292 MW
OMIM
X98429 mRNA
3D Structure
ModBase 3D Structure for Q92835
Immunogen
A synthesized peptide derived from human SHIP
Subcellular Localization
Cytoplasm. Cell membrane; Peripheral membrane protein. Membrane raft. Cytoplasm, cytoskeleton. Membrane; Peripheral membrane protein. Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Translocates from the cytoplasm to membrane ruffles in a FCGR3/CD16-dependent manner. Colocalizes with FC-gamma-RIIB receptor (FCGR2B) or FCGR3/CD16 at membrane ruffles. Tyrosine phosphorylation may also participate in membrane localization.
Tissue Specificity
Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level).
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-SHIP antibody

Immunology; Adaptive Immunity; T Cells; cytotoxic cells; Signal Transduction; Signaling Pathway; Lipid Signaling; lipid Phosphatases
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SHIP antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-SHIP antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IP: 1:50
FC/FACS: 1:50

Western Blot (WB) of anti-SHIP antibody

Western blot analysis of SHIP1 expression in Daudi cell lysate (MBS179954).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP5D monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for INPP5D
Rabbit Anti-SHIP Monoclonal Antibody – Cat# MBS179954

Immunohistochemistry (IHC) of anti-SHIP antibody

Immunohistochemical analysis of paraffin-embedded rat spleen, using SHIP Antibody(MBS179954)
INPP5D was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPP5D Antibody (MBS179954)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-SHIP Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS179954

Other Notes

Small volumes of anti-SHIP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SHIP purchase

MBS179954 is an antibody targeting SHIP. Also known as Anti-SHIP Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SHIP. It may not necessarily be applicable to this product.

NCBI GI #
64085167
NCBI GeneID
3635
NCBI Accession #
NP_001017915.1
UniProt Primary Accession #
Q92835
UniProt Secondary Accession #
O00145; Q13544; Q13545; Q6P5A4; Q92656; Q9UE80
UniProt Related Accession #
Q92835
GenBank Nucleotide #
NM_001017915.2
NCBI Official Full Name
phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform a
NCBI Official Synonym Full Names
inositol polyphosphate-5-phosphatase D
NCBI Official Symbol
INPP5D
NCBI Official Synonym Symbols
SHIP; SHIP1; SHIP-1; hp51CN; SIP-145; p150Ship
UniProt Protein Name
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
UniProt Synonym Protein Names
Inositol polyphosphate-5-phosphatase of 145 kDa; SIP-145; SH2 domain-containing inositol 5'-phosphatase 1; SH2 domain-containing inositol phosphatase 1; SHIP-1; p150Ship; hp51CN
Protein Family
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase
UniProt Gene Name
INPP5D
UniProt Synonym Gene Names
SHIP; SHIP1; SIP-145; SH2 domain-containing inositol phosphatase 1; SHIP-1; hp51CN
NCBI Protein Information
phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1

NCBI Summary for SHIP

This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in this gene are associated with defects and cancers of the immune system. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]
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UniProt Comments for SHIP

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.
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Research Articles on SHIP

1. JARID1B directly bound to PI3K/AKT signaling inhibitor SHIP1 gene promoter and decreased SHIP1 gene expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SHIP antibody

 Reference Product  PubMed Publications
 PLCG1 antibody  >1 publications with SHIP and PLCG1
 GRB2 antibody  >1 publications with SHIP and GRB2

Pathways associated with anti-SHIP antibody

 Products by Pathway  Pathway Diagram
 1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway antibodies  1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway Diagram
 1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway antibodies  1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway Diagram
 3-phosphoinositide Degradation Pathway antibodies  3-phosphoinositide Degradation Pathway Diagram
 3-phosphoinositide Degradation Pathway antibodies  3-phosphoinositide Degradation Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram

Diseases associated with anti-SHIP antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >3 publications with SHIP and Neoplasms
 Fibrosis Antibodies  >1 publications with SHIP and Fibrosis
 Carcinoma Antibodies  >1 publications with SHIP and Carcinoma
 Liver Cirrhosis Antibodies  >1 publications with SHIP and Liver Cirrhosis
 Liver Diseases Antibodies  >1 publications with SHIP and Liver Diseases
 Death Antibodies  >1 publications with SHIP and Death
 Inflammation Antibodies  >1 publications with SHIP and Inflammation

Organs/Tissues associated with anti-SHIP antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6 publications with SHIP and Blood
 Brain Antibodies  >1 publications with SHIP and Brain
 Lymph Antibodies  >1 publications with SHIP and Lymph
 Spleen Antibodies  >1 publications with SHIP and Spleen
 Lymph Node Antibodies  >1 publications with SHIP and Lymph Node
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