• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Calnexin (CANX) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179957

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Calnexin (CANX), Monoclonal Antibody
Full Product Name
Anti-Calnexin Rabbit Monoclonal Antibody
Product Gene Name
anti-CANX antibody
Product Synonym Names
Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90; CANX
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
67568 MW
OMIM
L10284 mRNA
3D Structure
ModBase 3D Structure for P27824
Immunogen
A synthesized peptide derived from human Calnexin
Subcellular Localization
Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed: 12643545, PubMed: 17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed: 22314232).
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CANX antibody

Tags & Cell Markers; Subcellular Markers; organelles; Neuroscience; Sensory System; visual system
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CANX antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-CANX antibody
WB: 1:1000-1:2000
IHC: 1:100-1:500
ICC/IF: 1:50-1:200
FC/FACS: 1:100

Western Blot (WB) of anti-CANX antibody

Western blot analysis of Calnexin expression in HepG2 cell lysate (MBS179957).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CANX monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CANX
Rabbit Anti-CANX Monoclonal Antibody – Cat# MBS179957

Other Notes

Small volumes of anti-CANX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CANX purchase

MBS179957 is an antibody targeting CANX. Also known as Anti-Calnexin Rabbit Monoclonal Antibody. Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CANX. It may not necessarily be applicable to this product.

NCBI GI #
66933005
NCBI GeneID
821
NCBI Accession #
NP_001019820.1
UniProt Primary Accession #
P27824
UniProt Secondary Accession #
B2R5V8; B4DGP8; B4E2T8; D3DWQ3; D6R9K3
UniProt Related Accession #
P27824
GenBank Nucleotide #
NM_001024649.1
NCBI Official Full Name
calnexin
NCBI Official Synonym Full Names
calnexin
NCBI Official Symbol
CANX
NCBI Official Synonym Symbols
CNX; P90; IP90
UniProt Protein Name
Calnexin
UniProt Synonym Protein Names
IP90; Major histocompatibility complex class I antigen-binding protein p88; p90
Protein Family
Calnexin
UniProt Gene Name
CANX
NCBI Protein Information
calnexin

NCBI Summary for CANX

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for CANX

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Show more

Research Articles on CANX

1. Increased tumour protein levels of calnexin may be of prognostic significance in colorectal cancer, and calnexin may represent a potential target for future therapies.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CANX antibody

 Reference Product  PubMed Publications
 HLA-A antibody  >1 publications with CANX and HLA-A
 PDIA3 antibody  >1 publications with CANX and PDIA3

Pathways associated with anti-CANX antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies  Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Assembly Of Viral Components At The Budding Site Pathway antibodies  Assembly Of Viral Components At The Budding Site Pathway Diagram
 Calnexin/calreticulin Cycle Pathway antibodies  Calnexin/calreticulin Cycle Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with anti-CANX antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >2 publications with CANX and Carcinoma
 Adenocarcinoma Antibodies  >2 publications with CANX and Adenocarcinoma
 Neoplasms Antibodies  >2 publications with CANX and Neoplasms
 Gastrointestinal Diseases Antibodies  >1 publications with CANX and Gastrointestinal Diseases
 Liver Diseases Antibodies  >1 publications with CANX and Liver Diseases

Organs/Tissues associated with anti-CANX antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >2 publications with CANX and Lung
 Liver Antibodies  >2 publications with CANX and Liver
 Testis Antibodies  >2 publications with CANX and Testis
 Epididymis Antibodies  >1 publications with CANX and Epididymis
 Blood Antibodies  >1 publications with CANX and Blood
 Kidney Antibodies  >1 publications with CANX and Kidney
 Skin Antibodies  >1 publications with CANX and Skin
 Uterus Antibodies  >1 publications with CANX and Uterus
 Embryonic Tissue Antibodies  >1 publications with CANX and Embryonic Tissue
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000