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RNF20 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179964

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4'… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RNF20, Monoclonal Antibody
Full Product Name
Anti-RNF20 Rabbit Monoclonal Antibody
Product Gene Name
anti-RNF20 antibody
Product Synonym Names
E3 ubiquitin-protein ligase BRE1A; BRE1-A; hBRE1; 6.3.2.-; RING finger protein 20; RNF20; BRE1A
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
113662 MW
OMIM
AF265230 mRNA
3D Structure
ModBase 3D Structure for Q5VTR2
Immunogen
A synthesized peptide derived from human RNF20
Subcellular Localization
Nucleus.
Tissue Specificity
Expressed in the normal brain and also in malignant gliomas (at protein level).
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-RNF20 antibody

Epigenetics and Nuclear Signaling; Chromatin Modifying Enzymes; ubiquitylation; Cell Biology; Proteolysis/Ubiquitin; Proteasome/Ubiquitin; ubiquitin e3 Enzymes; ring finger e3 ligase; Epigenetics and Nuclear Signaling; histones; h2b; ubiquitinated
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RNF20 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-RNF20 antibody
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50

Western Blot (WB) of anti-RNF20 antibody

Western blot analysis of RNF20 expression in (1) 293 cell lysate; (2) HeLa cell lysate (MBS179964).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF20 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RNF20
Rabbit Anti-RNF20 Monoclonal Antibody – Cat# MBS179964

Other Notes

Small volumes of anti-RNF20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RNF20 purchase

MBS179964 is an antibody targeting RNF20. Also known as Anti-RNF20 Rabbit Monoclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RNF20. It may not necessarily be applicable to this product.

NCBI GI #
34878777
NCBI GeneID
56254
NCBI Accession #
NP_062538.5
UniProt Primary Accession #
Q5VTR2
UniProt Secondary Accession #
Q2TB34; Q69YL5; Q6P527; Q8N3J4; Q96JD3; Q9H9Y7; Q9HA51; Q9NUR4; Q9NWQ3; Q9NX83; A7MCT5
UniProt Related Accession #
Q5VTR2
GenBank Nucleotide #
NM_019592.6
NCBI Official Full Name
E3 ubiquitin-protein ligase BRE1A
NCBI Official Synonym Full Names
ring finger protein 20
NCBI Official Symbol
RNF20
NCBI Official Synonym Symbols
BRE1; BRE1A; hBRE1
UniProt Protein Name
E3 ubiquitin-protein ligase BRE1A
UniProt Synonym Protein Names
RING finger protein 20; RING-type E3 ubiquitin transferase BRE1ACurated
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
RNF20
UniProt Synonym Gene Names
BRE1A; BRE1-A; hBRE1
NCBI Protein Information
E3 ubiquitin-protein ligase BRE1A

NCBI Summary for RNF20

The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). [provided by RefSeq, Feb 2012]
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Research Articles on RNF20

1. The authors also show that the RING domains of RNF20 and RNF40 can form a stable heterodimer that is active.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-RNF20 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >3 publications with RNF20 and Carcinoma
 Lung Diseases Antibodies  >1 publications with RNF20 and Lung Diseases
 Fatty Liver Antibodies  >1 publications with RNF20 and Fatty Liver
 Lung Neoplasms Antibodies  >1 publications with RNF20 and Lung Neoplasms
 Hypertrophy Antibodies  >1 publications with RNF20 and Hypertrophy
 Liver Diseases Antibodies  >1 publications with RNF20 and Liver Diseases
 Precancerous Conditions Antibodies  >1 publications with RNF20 and Precancerous Conditions

Organs/Tissues associated with anti-RNF20 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >3 publications with RNF20 and Lung
 Prostate Antibodies  >2 publications with RNF20 and Prostate
 Liver Antibodies  >2 publications with RNF20 and Liver
 Brain Antibodies  >2 publications with RNF20 and Brain
 Embryonic Tissue Antibodies  >1 publications with RNF20 and Embryonic Tissue
 Muscle Antibodies  >1 publications with RNF20 and Muscle
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