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Cubilin (CUBN) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179980

Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Cubilin (CUBN), Monoclonal Antibody
Full Product Name
Anti-Cubilin Rabbit Monoclonal Antibody
Product Gene Name
anti-CUBN antibody
Product Synonym Names
Cubilin; 460 kDa receptor; Intestinal intrinsic factor receptor; Intrinsic factor-cobalamin receptor; Intrinsic factor-vitamin B12 receptor; CUBN; IFCR
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
398736 MW
OMIM
AF034611 mRNA
3D Structure
ModBase 3D Structure for O60494
Immunogen
A synthesized peptide derived from human Cubilin
Subcellular Localization
Apical cell membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, coated pit. Endosome. Lysosome membrane; Peripheral membrane protein. Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells.
Tissue Specificity
Expressed in kidney proximal tubule cells, placenta, visceral yolk-sac cells and in absorptive intestinal cells. Expressed in the epithelium of intestine and kidney.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CUBN antibody

Signal Transduction; Metabolism; Vitamins/Minerals; Signal Transduction; Lipid Metabolism; Cancer; Cancer Metabolism; Metabolic Signaling Pathway; Metabolism of Lipids and Lipoproteins; Hormone BioSynthesis; Metabolism; Pathways and Processes; Metabolic S
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CUBN antibody
Western Blot (WB)
Application Notes for anti-CUBN antibody
WB: 1:500-1:2000

Western Blot (WB) of anti-CUBN antibody

Western blot analysis of Cubilin expression in Human fetal kidney lysate (MBS179980).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CUBN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CUBN
Rabbit Anti-CUBN Monoclonal Antibody – Cat# MBS179980

Other Notes

Small volumes of anti-CUBN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CUBN purchase

MBS179980 is an antibody targeting CUBN. Also known as Anti-Cubilin Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CUBN. It may not necessarily be applicable to this product.

NCBI GI #
126091152
NCBI GeneID
8029
NCBI Accession #
NP_001072.2
UniProt Primary Accession #
O60494
UniProt Secondary Accession #
Q5VTA6; Q96RU9; B0YIZ4
UniProt Related Accession #
O60494
GenBank Nucleotide #
NM_001081.3
NCBI Official Full Name
cubilin
NCBI Official Synonym Full Names
cubilin
NCBI Official Symbol
CUBN
NCBI Official Synonym Symbols
IFCR; MGA1; gp280
UniProt Protein Name
Cubilin
UniProt Synonym Protein Names
460 kDa receptor; Intestinal intrinsic factor receptor; Intrinsic factor-cobalamin receptor; Intrinsic factor-vitamin B12 receptor
Protein Family
Cubilin
UniProt Gene Name
CUBN
UniProt Synonym Gene Names
IFCR
NCBI Protein Information
cubilin

NCBI Summary for CUBN

Cubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. The role of receptor is supported by the presence of 27 CUB domains. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia. [provided by RefSeq, Jul 2008]
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Research Articles on CUBN

1. Study identified CUBN as a marker for risk stratification of patients with renal cell carcinoma (RCC). Lack of CUBN expression was significantly associated with early disease progression and poor patient outcome, independent of T-stage, Fuhrman grade and nodal status. Owing to a highly RCC-specific expression profile, CUBN expression also has a potential role in clinical cancer differential diagnostics.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CUBN antibody

 Reference Product  PubMed Publications
 AMN antibody  >21 publications with CUBN and AMN
 LRP2 antibody  >7 publications with CUBN and LRP2
 GIF antibody  >6 publications with CUBN and GIF
 CBL antibody  >5 publications with CUBN and CBL

Pathways associated with anti-CUBN antibody

 Products by Pathway  Pathway Diagram
 Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway antibodies  Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway Diagram
 Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defects In Cobalamin (B12) Metabolism Pathway antibodies  Defects In Cobalamin (B12) Metabolism Pathway Diagram
 Defects In Vitamin And Cofactor Metabolism Pathway antibodies  Defects In Vitamin And Cofactor Metabolism Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Of Metabolism Pathway antibodies  Diseases Of Metabolism Pathway Diagram
 HDL-mediated Lipid Transport Pathway antibodies  HDL-mediated Lipid Transport Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram

Diseases associated with anti-CUBN antibody

 Disease Name  Pubmed Publications
 Proteinuria Antibodies  >30 publications with CUBN and Proteinuria
 Anemia Antibodies  >26 publications with CUBN and Anemia
 MEGALOBLASTIC ANEMIA 1 Antibodies  >18 publications with CUBN and MEGALOBLASTIC ANEMIA 1
 Kidney Diseases Antibodies  >7 publications with CUBN and Kidney Diseases
 Congenital Abnormalities Antibodies  >6 publications with CUBN and Congenital Abnormalities
 Heart Diseases Antibodies  >5 publications with CUBN and Heart Diseases
 Disease Models, Animal Antibodies  >5 publications with CUBN and Disease Models, Animal
 Renal Insufficiency Antibodies  >4 publications with CUBN and Renal Insufficiency
 Inflammation Antibodies  >2 publications with CUBN and Inflammation
 Hypertension Antibodies  >1 publications with CUBN and Hypertension

Organs/Tissues associated with anti-CUBN antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >21 publications with CUBN and Kidney
 Intestine Antibodies  >5 publications with CUBN and Intestine
 Bone Antibodies  >3 publications with CUBN and Bone
 Lung Antibodies  >3 publications with CUBN and Lung
 Embryonic Tissue Antibodies  >2 publications with CUBN and Embryonic Tissue
 Brain Antibodies  >2 publications with CUBN and Brain
 Pancreas Antibodies  >1 publications with CUBN and Pancreas
 Eye Antibodies  >1 publications with CUBN and Eye
 Testis Antibodies  >1 publications with CUBN and Testis
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