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Helios (IKZF2) Antibody – Hamster Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800089

Helios, also known as IKZF2 (Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo-… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Helios (IKZF2), Monoclonal Antibody
Full Product Name
Anti-Helios PE
Product Gene Name
Product Synonym Names
IKAROS family zinc finger 2; ANF1A2; IKZF2
Clonality
Monoclonal
Isotype
IgG
Clone Number
22F6
Host
Armenian Hamster
Species Reactivity
Mouse, Human
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Purity/Purification
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Specificity
The Armenian hamster monoclonal antibody 22F6 recognizes Helios, a transcription factor (intracellular antigen) expressed in some hematopoietic stem cells, and at high levels in thymic-derived regulatory T cells. The epitope is located between amino acids 51 and 107.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Matching Pairs
Unconjugated Antibody: Helios Clone #22F6 (MBS1800088)
PE Conjugated Antibody: Helios Clone #22F6 (MBS1800089)
OMIM
606234
3D Structure
ModBase 3D Structure for Q9UKS7
Immunogen
Peptide coresponding to the amino acids 51-107 of Helios
Conjugation
PE
Applications Tested/Suitable for anti-IKZF2 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-IKZF2 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-IKZF2 antibody

Human peripheral blood lymphocytes were stained with anti-CD4 Pacific BlueTM, fixed and permeabilized with Treg Detection Kit (Miltenyi cat. 130-094-163), and then intracellulary stained with anti-Helios PE (clone 22F6).
Hamster Anti-IKZF2 Monoclonal Antibody – Cat# MBS1800089

Other Notes

Small volumes of anti-IKZF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IKZF2 purchase

MBS1800089 is an antibody targeting IKZF2. Also known as Anti-Helios PE. Reactive in Mouse, Human. Host: Armenian Hamster.

Additional Details

NCBI/Uniprot data below describe general gene information for IKZF2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
zinc finger protein Helios isoform 2
NCBI Official Synonym Full Names
IKAROS family zinc finger 2
NCBI Official Symbol
IKZF2
NCBI Official Synonym Symbols
ANF1A2; HELIOS; ZNF1A2; ZNFN1A2
UniProt Protein Name
Zinc finger protein Helios
UniProt Synonym Protein Names
Ikaros family zinc finger protein 2
Protein Family
UniProt Gene Name
IKZF2
UniProt Synonym Gene Names
HELIOS; ZNFN1A2
UniProt Entry Name
IKZF2_HUMAN
NCBI Protein Information
zinc finger protein Helios

NCBI Summary for IKZF2

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for IKZF2

Helios: Associates with Ikaros at centromeric heterochromatin. Belongs to the Ikaros C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; metal ion binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IKZF2 antibody

 Reference Product  PubMed Publications
 IKZF1 antibody  >9 publications with IKZF2 and IKZF1
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