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CD99 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800152

CD99 is a ubiquitous transmembrane type I sialoglycoprotein of a unique and poorly characterized protein family. CD99 is heavily O-glycosylated and was described as a T cell costimulator and strong activator of integrin-mediated actin cytoskeleton assembly,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD99, Monoclonal Antibody
Full Product Name
Anti-Hu CD99 PE-Cyanine7
Product Gene Name
Product Synonym Names
CD99 molecule (Xg blood group); MIC2; HBA71; blood group Xg
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
3B2/TA8
Host
Mouse
Species Reactivity
Human
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Purity/Purification
The purified antibody is conjugated with tandem dye PE-Cyanine7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Specificity
The mouse monoclonal antibody 3B2/TA8 recognizes CD99, an approximately 32 kDa sialoglycoprotein expressed on the surface of many cell types, with particularly strong expression on Ewing's sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Matching Pairs
Unconjugated Antibody: CD99 Clone #3B2/TA8 (MBS1800148)
PE-CY7 Conjugated Antibody: CD99 Clone #3B2/TA8 (MBS1800152)
OMIM
313470
3D Structure
ModBase 3D Structure for P14209
Immunogen
Human thymocytes
Conjugation
PE-Cyanine7
Applications Tested/Suitable for anti-CD99 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD99 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-CD99 antibody

Separation of human CD99 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD99 (3B2/TA8) PE-Cy™7 antibody (4 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-CD99 Monoclonal Antibody – Cat# MBS1800152

Flow Cytometry (FC/FACS) of anti-CD99 antibody

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD99 (3B2/TA8) PE-Cy™7 antibody (4 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-CD99 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800152

Other Notes

Small volumes of anti-CD99 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD99 purchase

MBS1800152 is an antibody targeting CD99. Also known as Anti-Hu CD99 PE-Cyanine7. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD99. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD99 antigen isoform a
NCBI Official Synonym Full Names
CD99 molecule (Xg blood group)
NCBI Official Symbol
CD99
NCBI Official Synonym Symbols
MIC2; HBA71; MIC2X; MIC2Y; MSK5X
UniProt Protein Name
CD99 antigen
UniProt Synonym Protein Names
12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2
Protein Family
UniProt Gene Name
CD99
UniProt Synonym Gene Names
MIC2; MIC2X; MIC2Y
UniProt Entry Name
CD99_HUMAN
NCBI Protein Information
CD99 antigen

NCBI Summary for CD99

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]
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UniProt Comments for CD99

CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3

Cellular Component: focal adhesion; integral to plasma membrane; cytoplasm; plasma membrane

Biological Process: cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD99 antibody

 Reference Product  PubMed Publications
 EWSR1 antibody  >86 publications with CD99 and EWSR1
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