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CD137 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800187

CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells.… More details

$385 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD137, Monoclonal Antibody
Full Product Name
Anti-Hu CD137 APC
Product Gene Name
Product Synonym Gene Name
TNFRSF9
Product Synonym Names
TNF receptor superfamily member 9; TNFRSF9; 41BB; 4-1BB; MGC2172
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
4B4-1
Host
Mouse
Species Reactivity
Human, Non-human primates
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Purity/Purification
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Specificity
The mouse monoclonal antibody 4B4-1 recognizes an extracellular conformational epitope on CD137, an approximately 40 kDa type I transmembrane protein of the TNFR family expressed mainly on activated T cells.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Matching Pairs
Unconjugated Antibody: CD137 Clone #4B4-1 (MBS1800185)
APC Conjugated Antibody: CD137 Clone #4B4-1 (MBS1800187)
OMIM
602250
3D Structure
ModBase 3D Structure for Q07011
Immunogen
Recombinant human CD137 ectodomain
Conjugation
APC
Applications Tested/Suitable for anti-CD137 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD137 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-CD137 antibody

Flow cytometry multicolor surface staining of human peripheral blood mononulcear cells stained using anti-humam CD137 (4B4-1) APC antibody (10 ul reagent per milion cells in 100 ul of cell suspension) and anti-human CD25 (MEM-181) PE antibody (20 ul reagent per milion cells in 100 ul of cell suspension).
Mouse Anti-CD137 Monoclonal Antibody – Cat# MBS1800187

Flow Cytometry (FC/FACS) of anti-CD137 antibody

Separation of cells stained using anti-human CD137 (4B4-1) APC antibody (10 ul reagent per milion cells in 100 ul of cell suspension, red-filled) from cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 1 ug/ml, same as CD137 APC concentration, black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cell suspension.
Mouse Anti-CD137 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800187

Flow Cytometry (FC/FACS) of anti-CD137 antibody

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD137 (4B4-1) APC antibody (10 ul reagent per milion cells in 100 ul of cell suspension).
Mouse Anti-CD137 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800187

Other Notes

Small volumes of anti-CD137 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD137 purchase

MBS1800187 is an antibody targeting CD137. Also known as Anti-Hu CD137 APC. Reactive in Human, Non-human primates. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD137. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 9
NCBI Official Synonym Full Names
TNF receptor superfamily member 9
NCBI Official Symbol
TNFRSF9
NCBI Official Synonym Symbols
ILA; 4-1BB; CD137; CDw137
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 9
UniProt Synonym Protein Names
4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA
UniProt Gene Name
TNFRSF9
UniProt Synonym Gene Names
CD137; ILA
UniProt Entry Name
TNR9_HUMAN
NCBI Protein Information
tumor necrosis factor receptor superfamily member 9

NCBI Summary for CD137

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD137

TNFRSF9: Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane

Molecular Function: cytokine binding; receptor activity

Biological Process: negative regulation of cell proliferation; apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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