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CD123 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800282

CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD123, Monoclonal Antibody
Full Product Name
Anti-Hu CD123 PE
Product Gene Name
Product Synonym Gene Name
IL3RA
Product Synonym Names
interleukin 3 receptor subunit alpha; IL3RA; interleukin 3 receptor alpha; MGC34174; hIL-3Ra
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6H6
Host
Mouse
Species Reactivity
Human
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Purity/Purification
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Specificity
The mouse monoclonal antibody 6H6 recognizes an extracellular epitope of CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Matching Pairs
Unconjugated Antibody: CD123 Clone #6H6 (MBS1800278)
PE Conjugated Antibody: CD123 Clone #6H6 (MBS1800282)
OMIM
gene 430000
3D Structure
ModBase 3D Structure for P26951
Immunogen
IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells
Conjugation
PE
Applications Tested/Suitable for anti-CD123 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD123 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-CD123 antibody

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD123 (6H6) PE antibody (10 ul reagent / 100 ul of peripheral whole blood) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (10 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-CD123 Monoclonal Antibody – Cat# MBS1800282

Flow Cytometry (FC/FACS) of anti-CD123 antibody

Separation of human CD123 positive Basophil granulocytes(red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD123 (6H6) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-CD123 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800282

Flow Cytometry (FC/FACS) of anti-CD123 antibody

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD123 (6H6) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-CD123 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800282

Other Notes

Small volumes of anti-CD123 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD123 purchase

MBS1800282 is an antibody targeting CD123. Also known as Anti-Hu CD123 PE. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD123. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Interleukin-3 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 3 receptor subunit alpha
NCBI Official Symbol
IL3RA
NCBI Official Synonym Symbols
IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
UniProt Protein Name
Interleukin-3 receptor subunit alpha
UniProt Synonym Protein Names
CD_antigen: CD123
UniProt Gene Name
IL3RA
UniProt Synonym Gene Names
IL3R; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA
UniProt Entry Name
IL3RA_HUMAN
NCBI Protein Information
interleukin-3 receptor subunit alpha

NCBI Summary for CD123

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012]
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UniProt Comments for CD123

IL3RA: This is a receptor for interleukin-3. Belongs to the type I cytokine receptor family. Type 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.3 or Yp11.3

Cellular Component: plasma membrane; integral to membrane

Molecular Function: interleukin-3 receptor activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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