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Cytokeratin 8 (KRT8) Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800477

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 8 (KRT8), Antibody
Full Product Name
Anti-Cytokeratin 8 FITC
Product Gene Name
Product Synonym Names
keratin 8; K8; CK8; CK-8; CYK8; KRT8; K2C8; CARD2
Isotype
IgG1
Clone Number
C-43
Host
Mouse
Species Reactivity
Cow, Human, Rabbit, Pig, Sheep
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Purity/Purification
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Concentration
1 mg/ml (lot specific)
Specificity
The antibody C-43 reacts with Cytokeratin 8 (52.5 kDa intracellular antigen). Cytokeratins are members of intermediate filaments subfamily represented in epithelial tissues.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Matching Pairs
3D Structure
ModBase 3D Structure for Q7L4M3
Immunogen
Cytoskeleton preparation from HeLa human cervix carcinoma cell line.
Negative Species
Mouse, Rat, Xenopus, Hamster, Chicken
Conjugation
FITC
Applications Tested/Suitable for anti-KRT8 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-KRT8 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-KRT8 antibody

Separation of A431 cellular suspension (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood mixed with A431 cellular suspension stained using anti-Cytokeratin 8 (C-43) FITC antibody (concentration in sample 9 ug/ml).
Mouse Anti-KRT8 Antibody – Cat# MBS1800477

Flow Cytometry (FC/FACS) of anti-KRT8 antibody

Flow cytometry intracellular staining pattern of human peripheral whole blood mixed with A431 cellular suspension stained using anti-Cytokeratin 8 (C-43) FITC antibody (concentration in sample 9 ug/ml).
Mouse Anti-KRT8 Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800477

Other Notes

Small volumes of anti-KRT8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT8 purchase

MBS1800477 is an antibody targeting KRT8. Also known as Anti-Cytokeratin 8 FITC. Reactive in Cow, Human, Rabbit, Pig, Sheep. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
KRT8 protein
NCBI Official Synonym Full Names
keratin 8
NCBI Official Symbol
KRT8
NCBI Official Synonym Symbols
K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Protein Family
NCBI Protein Information
keratin, type II cytoskeletal 8

NCBI Summary for KRT8

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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