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CD279 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800580

CD279 / PD-1 (programmed cell death 1), a transmembrane protein of CD28/CTLA-4 family. It is expressed inducibly mainly on activated T, B, and myeloid cells and plays a role in maintaining peripheral self-tolerance. Binding to its receptors CD273 and CD274 is… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD279, Monoclonal Antibody
Full Product Name
Anti-Hu CD279 PerCP-Cyanine5.5
Product Gene Name
Product Synonym Gene Name
PDCD1
Product Synonym Names
programmed cell death 1; PDCD1; PD1; PD-1; SLEB2
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
EH12.2H7
Host
Mouse
Species Reactivity
Non-human primates, Human
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Purity/Purification
The purified antibody is conjugated with tandem dye PerCP-Cyanine5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Specificity
The mouse monoclonal antibody EH12.2H7 recognizes an extracellular epitope of CD279 / PD-1 (programmed cell death 1), a 55 kDa type I transmembrane protein expressed above all during T cell development, on activated T cells, activated B cells, and activated monocytes.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Matching Pairs
Unconjugated Antibody: CD279 Clone #EH12.2H7 (MBS1800574)
PerCP-CY5.5 Conjugated Antibody: CD279 Clone #EH12.2H7 (MBS1800580)
OMIM
L27440 Genomic DNA
3D Structure
ModBase 3D Structure for Q15116
Conjugation
PerCP-Cyanine5.5
Applications Tested/Suitable for anti-CD279 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD279 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-CD279 antibody

Separation of human CD279 positive lymphocytes (red-filled) from CD279 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral whole blood stained using anti-human CD279 (BNI3) PerCP-Cy™5.5 antibody (4 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-CD279 Monoclonal Antibody – Cat# MBS1800580

Flow Cytometry (FC/FACS) of anti-CD279 antibody

Flow cytometry surface staining pattern of human PHA stimulated peripheral whole blood stained using anti-human CD279 (BNI3) PerCP-Cy™5.5 antibody (4 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-CD279 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800580

Other Notes

Small volumes of anti-CD279 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD279 purchase

MBS1800580 is an antibody targeting CD279. Also known as Anti-Hu CD279 PerCP-Cyanine5.5. Reactive in Non-human primates, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD279. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
PDCD1
NCBI Official Synonym Symbols
PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
UniProt Protein Name
Programmed cell death protein 1
UniProt Synonym Protein Names
CD_antigen: CD279
UniProt Gene Name
PDCD1
UniProt Synonym Gene Names
PD1; Protein PD-1; hPD-1
NCBI Protein Information
programmed cell death protein 1

NCBI Summary for CD279

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD279

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-CD279 antibody

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