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CD9 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800583

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and… More details

$265 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD9, Monoclonal Antibody
Full Product Name
Anti-Ms CD9 Biotin
Product Gene Name
Product Synonym Names
CD9 molecule; Mic3; Mrp-1; Btcc-1; Drap-27; Tspan-29
Clonality
Monoclonal
Isotype
IgG1
Clone Number
EM-04
Host
Rat
Species Reactivity
Mouse
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Purity/Purification
The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Concentration
1 mg/ml (lot specific)
Specificity
The rat monoclonal antibody EM-04 recognizes an extracellular epitope of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
Preparation and Storage
Store at 2-8 degree C.
Matching Pairs
Unconjugated Antibody: CD9 Clone #EM-04 (MBS1800581)
Biotin Conjugated Antibody: CD9 Clone #EM-04 (MBS1800583)
3D Structure
ModBase 3D Structure for P40240
Immunogen
Permeabilized murine bone marrow-derived mast cells (BMMC).
Conjugation
Biotin
Applications Tested/Suitable for anti-CD9 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-CD9 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-CD9 antibody

Flow cytometry multicolor surface staining pattern of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 ug/ml, Streptavidin APC) and SYTO16.
Rat Anti-CD9 Monoclonal Antibody – Cat# MBS1800583

Flow Cytometry (FC/FACS) of anti-CD9 antibody

Separation of murine myeloid cells (red-filled) from CD9 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 ug/ml, Streptavidin APC).
Rat Anti-CD9 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800583

Flow Cytometry (FC/FACS) of anti-CD9 antibody

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 ug/ml, Streptavidin APC).
Rat Anti-CD9 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800583

Other Notes

Small volumes of anti-CD9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD9 purchase

MBS1800583 is an antibody targeting CD9. Also known as Anti-Ms CD9 Biotin. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD9. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD9 antigen
NCBI Official Synonym Full Names
CD9 antigen
NCBI Official Symbol
Cd9
NCBI Official Synonym Symbols
Tspan29
UniProt Protein Name
CD9 antigen
UniProt Synonym Protein Names
CD_antigen: CD9
Protein Family
UniProt Gene Name
Cd9
UniProt Entry Name
CD9_MOUSE
NCBI Protein Information
CD9 antigen

UniProt Comments for CD9

CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Membrane protein, multi-pass

Cellular Component: apical plasma membrane; cell surface; external side of plasma membrane; extracellular space; focal adhesion; integral to plasma membrane; membrane; plasma membrane

Molecular Function: integrin binding; protein binding

Biological Process: cell adhesion; cell motility; cell surface receptor linked signal transduction; fusion of sperm to egg plasma membrane; negative regulation of cell proliferation; paranodal junction assembly; receptor internalization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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