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CD45 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800586

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte… More details

$250 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD45, Monoclonal Antibody
Full Product Name
Anti-Ms CD45 Purified
Product Gene Name
anti-CD45 antibody
Product Synonym Gene Name
Ptprc
Product Synonym Names
protein tyrosine phosphatase, receptor type, C; LCA; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; LY5; GP180
Clonality
Monoclonal
Isotype
IgG
Clone Number
EM-05
Host
Rat
Species Reactivity
Mouse
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Purity/Purification
Purified by Protein-A Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
The rat monoclonal antibody EM-05 reacts with an extracellular epitope of murine CD45 antigen (Leukocyte Common Antigen), a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.
Preparation and Storage
Store at 2-8 degree C.
Matching Pairs
Unconjugated Antibody: CD45 Clone #EM-05 (MBS1800586)
APC Conjugated Antibody: CD45 Clone #EM-05 (MBS1800587)
Matching Pairs
Unconjugated Antibody: CD45 Clone #EM-05 (MBS1800586)
PE Conjugated Antibody: CD45 Clone #EM-05 (MBS1800588)
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3D Structure
ModBase 3D Structure for Q8C6Q7
Immunogen
Murine peripheral blood leukocytes
Applications Tested/Suitable for anti-CD45 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-CD45 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-CD45 antibody

Separation of murine splenocytes stained using anti-mouse CD45 (EM-05) purified antibody (concentration in sample 1.67 ug/ml, DAR APC, red-filled) from murine splenocytes unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension.
Rat Anti-CD45 Monoclonal Antibody – Cat# MBS1800586

Flow Cytometry (FC/FACS) of anti-CD45 antibody

Flow cytometry surface staining pattern of murine splenocytes stained using anti-mouse CD45 (EM-05) purified antibody (concentration in sample 1.67 ug/ml, DAR APC).
Rat Anti-CD45 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800586

Other Notes

Small volumes of anti-CD45 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD45 purchase

MBS1800586 is an antibody targeting CD45. Also known as Anti-Ms CD45 Purified. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD45. It may not necessarily be applicable to this product.

NCBI GI #
123795742
NCBI GeneID
19264
UniProt Primary Accession #
Q8C6Q7
UniProt Related Accession #
P06800
NCBI Official Full Name
2 days pregnant adult female oviduct cDNA, RIKEN full-length enriched library, clone:E230015G23 product:protein tyrosine phosphatase, receptor type, C, full insert sequence
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol
Ptprc
NCBI Official Synonym Symbols
loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
Protein Family
Protein tyrosine phosphatase receptor type C-associated protein
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C

Research Articles on CD45

1. PTPdelta D2 interacts with the N-terminal helix and the first and second SAMs (SAM1 and SAM2, respectively) of Liprin-alpha3.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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