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N-Sulfated Heparan Sulfate Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800696

MBS1800696 is an antibody targeting N-Sulfated Heparan Sulfate. Also known as Anti-N-Sulfated Heparan Sulfate PE. Reactive in General. Host: Mouse. More details

$400 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
N-Sulfated Heparan Sulfate, Monoclonal Antibody
Full Product Name
Anti-N-Sulfated Heparan Sulfate PE
Product Synonym Names
N-sulfo-rich heparan sulfate
Clonality
Monoclonal
Isotype
IgM
Clone Number
HepSS-1
Host
Mouse
Species Reactivity
General
Form/Format
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Purity/Purification
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Concentration
0.1 mg/ml (lot specific)
Specificity
The mouse monoclonal antibody HepSS-1 (also known as HepSS1) recognizes N-sulfated heparan sulfate (axtracellular antigen) present in many species.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Matching Pairs
Unconjugated Antibody: N-sulfated heparan sulfate Clone #HepSS-1 (MBS1800694)
PE Conjugated Antibody: N-sulfated heparan sulfate Clone #HepSS-1 (MBS1800696)
Immunogen
MethA murine fibrosarcoma
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography
Conjugation
PE
Antigen
N-sulfated heparan sulfate
Excitation laser
blue (488 nm)
Applications Tested/Suitable for anti-N-Sulfated Heparan Sulfate antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-N-Sulfated Heparan Sulfate antibody
Flow Cytometry (FC): 2-5 ug/ml

Flow Cytometry (FC/FACS) of anti-N-Sulfated Heparan Sulfate antibody

Separation of EA.hy926 cells (red-filled) from EA.hy926 cells treated with heparinase (1 hour, 1 UI per ml of cell suspension) stained using anti-N-sulfated heparan sulfate (HepSS-1) PE antibody (concentration in sample 10 ug/ml).
Mouse Anti-N-Sulfated Heparan Sulfate Monoclonal Antibody – Cat# MBS1800696

Other Notes

Small volumes of anti-N-Sulfated Heparan Sulfate antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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