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ACTIN Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800697

Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of… More details

$345 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Actin, Monoclonal Antibody
Full Product Name
Anti-Actin (muscle) Purified
Product Gene Name
Product Synonym Names
Muscle specific actin
Clonality
Monoclonal
Isotype
IgG1
Clone Number
HHF35
Host
Mouse
Species Reactivity
Human, Chicken, Mouse, Non-human primates, Dog, Rat, Rabbit, Cat
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Purity/Purification
Purified by Protein-A Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.
Preparation and Storage
Store at 2-8 degree C.
3D Structure
ModBase 3D Structure for P60706
Immunogen
SDS extracted protein fraction of human myocardium
Applications Tested/Suitable for anti-Actin antibody
Western Blot (WB), Immunohistochemistry (IHC) Frozen/Paraffin

Western Blot (WB) of anti-Actin antibody

Western blotting analysis of muscle-specific actin using the monoclonal antibody HHF35.
Lane 1: murine femoral muscle
Lane 2: murine heart
Lane 3: HeLa
Lane 4: HUVEC
Mouse Anti-ACTIN Monoclonal Antibody – Cat# MBS1800697

Immunohistochemistry (IHC)-Paraffin of anti-Actin antibody

Immunohistochemistry staining of human muscle (paraffin-embedded sections) with anti-muscle-specific actin (HHF35). Commercially tested by LifeSpan BioSciences.
Mouse Anti-ACTIN Monoclonal Antibody, Immunohistochemistry (IHC)-Paraffin – Cat# MBS1800697

Other Notes

Small volumes of anti-Actin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Actin purchase

MBS1800697 is an antibody targeting Actin. Also known as Anti-Actin (muscle) Purified. Reactive in Human, Chicken, Mouse, Non-human primates, Dog, Rat, Rabbit, Cat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Actin. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
Bact; actin
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actin
Protein Family
UniProt Gene Name
ACTB
NCBI Protein Information
actin, cytoplasmic 1

UniProt Comments for Actin

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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