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CD235a Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800816

CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between… More details

$385 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD235a, Monoclonal Antibody
Full Product Name
Anti-Hu CD235a APC
Product Gene Name
Product Synonym Gene Name
GYPA
Product Synonym Names
glycophorin A (MNS blood group); Glycophorin A; GYPA; GPA; PAS-2; Sialoglycoprotein alpha; MN sialoglycoprotein; GPSAT; GPErik; MSN blood group
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
JC159
Host
Mouse
Species Reactivity
Rat, Human
Form/Format
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Specificity
The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.
Preparation and Storage
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Matching Pairs
Unconjugated Antibody: CD235a Clone #JC159 (MBS1800815)
APC Conjugated Antibody: CD235a Clone #JC159 (MBS1800816)
OMIM
M12857 mRNA
3D Structure
ModBase 3D Structure for P02724
Antigen
CD235a
Immunogen
Membrane preparation from splenic hairy cell leukemia
Conjugation
APC
Excitation Laser
red (633nm)
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Applications Tested/Suitable for anti-CD235a antibody
Flow Cytometry (FC)
Application Notes for anti-CD235a antibody
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10ul reagent/ 100 ul of whole blood or 106 cells n a suspension. The content of a vial 1(ml) is sufficient for 100 tests.

Other Notes

Small volumes of anti-CD235a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD235a purchase

MBS1800816 is an antibody targeting CD235a. Also known as Anti-Hu CD235a APC. Reactive in Rat, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD235a. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Glycophorin-A
NCBI Official Synonym Full Names
glycophorin A (MNS blood group)
NCBI Official Symbol
GYPA
NCBI Official Synonym Symbols
MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
UniProt Protein Name
Glycophorin-A
UniProt Synonym Protein Names
MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; CD_antigen: CD235a
Protein Family
UniProt Gene Name
GYPA
UniProt Synonym Gene Names
GPA
NCBI Protein Information
glycophorin-A

NCBI Summary for CD235a

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD235a

Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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