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Ki-67 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800829

Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ki-67, Monoclonal Antibody
Full Product Name
Anti-Ki-67 PE
Product Gene Name
Product Synonym Gene Name
MKI67
Product Synonym Names
marker of proliferation Ki-67; KIA; MIB; Marker of proliferation Ki67; MKI67
Clonality
Monoclonal
Isotype
IgG1
Clone Number
Ki-67
Host
Mouse
Species Reactivity
Cow, Human
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Purity/Purification
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Specificity
The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Matching Pairs
Unconjugated Antibody: Ki-67 Clone #Ki-67 (MBS1800828)
PE Conjugated Antibody: Ki-67 Clone #Ki-67 (MBS1800829)
OMIM
176741
3D Structure
ModBase 3D Structure for P46013
Immunogen
Nuclei of the Hodgkin lymphoma cell line L428
Conjugation
PE
Applications Tested/Suitable for anti-Ki-67 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-Ki-67 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-Ki-67 antibody

Flow cytometry analysis of human peripheral blood mononuclear cells stimulated with PHA. Surface staining of CD25 (clone MEM-181 APC) was followed by permeabilization and nuclear staining of Ki-67 (clone Ki-67 PE).
Mouse Anti-Ki-67 Monoclonal Antibody – Cat# MBS1800829

Other Notes

Small volumes of anti-Ki-67 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ki-67 purchase

MBS1800829 is an antibody targeting Ki-67. Also known as Anti-Ki-67 PE. Reactive in Cow, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Ki-67. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
proliferation marker protein Ki-67 isoform 2
NCBI Official Synonym Full Names
marker of proliferation Ki-67
NCBI Official Symbol
MKI67
NCBI Official Synonym Symbols
KIA; MIB-; MIB-1; PPP1R105
UniProt Protein Name
Antigen KI-67
UniProt Gene Name
MKI67
UniProt Entry Name
KI67_HUMAN
NCBI Protein Information
proliferation marker protein Ki-67

NCBI Summary for Ki-67

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

UniProt Comments for Ki-67

KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.

Protein type: Nucleolus; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q26.2

Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein C-terminus binding; protein binding; ATP binding

Biological Process: cell proliferation; meiosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Ki-67 antibody

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