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CD11b Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800928

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18… More details

$250 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD11b, Monoclonal Antibody
Full Product Name
Anti-Ms CD11b Purified Low Endotoxin
Product Gene Name
Product Synonym Gene Name
Itgam
Product Synonym Names
integrin subunit alpha M; Mac-1; Integrin alpha M; ITGAM; CR3A; MO1A; MAC1A
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
M1/70
Host
Rat
Species Reactivity
Mouse, Rabbit, Human, Non-human primates
Form/Format
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
Purity/Purification
Purified by Protein-G Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
The rat monoclonal antibody M1/70 detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
Preparation and Storage
Store at 2-8 degree C.
Matching Pairs
Unconjugated Antibody: CD11b Clone #M1/70 (MBS1800927)
Unconjugated Low Endotoxin Antibody: CD11b Clone #M1/70 (MBS1800928)
3D Structure
ModBase 3D Structure for G5E8F1
Immunogen
B10 mouse spleen cells enriched for T cells
Applications Tested/Suitable for anti-CD11b antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC) Frozen, Functional
Application Notes for anti-CD11b antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-CD11b antibody

Separation of murine CD11b positive myeloid cells (red-filled) from murine CD11b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD11b (M1/70) purified antibody (low endotoxin, concentration in sample 0,6 ug/ml) DAR APC.
Rat Anti-CD11b Monoclonal Antibody – Cat# MBS1800928

Flow Cytometry (FC/FACS) of anti-CD11b antibody

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD11b (M1/70) purified antibody (low endotoxin, concentration in sample 0,6 ug/ml) DAR APC.
Rat Anti-CD11b Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800928

Other Notes

Small volumes of anti-CD11b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD11b purchase

MBS1800928 is an antibody targeting CD11b. Also known as Anti-Ms CD11b Purified Low Endotoxin. Reactive in Mouse, Rabbit, Human, Non-human primates. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD11b. It may not necessarily be applicable to this product.

NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Synonym Full Names
integrin alpha M
NCBI Official Symbol
Itgam
NCBI Official Synonym Symbols
CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Protein Family
NCBI Protein Information
integrin alpha-M

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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