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CD50 Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800967

CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that… More details

$295 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD50, Antibody
Full Product Name
Anti-Hu CD50 Purified Low Endotoxin
Product Gene Name
Product Synonym Gene Name
ICAM3
Product Synonym Names
intercellular adhesion molecule 3; ICAM-3; ICAM-R
Isotype
IgG1
Clone Number
MEM-04
Host
Mouse
Species Reactivity
Human
Form/Format
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
Purity/Purification
Purified by Protein-A Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
The antibody MEM-04 reacts with an extracellular epitope of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes. The MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).
Preparation and Storage
Store at 2-8 degree C.
OMIM
146631
3D Structure
ModBase 3D Structure for P32942
Immunogen
Leukocytes of a patient suffering from LGL-type leukemia.
Applications Tested/Suitable for anti-CD50 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Functional
Application Notes for anti-CD50 antibody
FC/FACS: QC tested

Immunohistochemistry (IHC)-Paraffin of anti-CD50 antibody

Immunohistochemistry staining of human tonsil (paraffin sections) using anti-CD50 (MEM-04).
Commercially tested by LifeSpan BioSciences.
Mouse Anti-CD50 Antibody – Cat# MBS1800967

Flow Cytometry (FC/FACS) of anti-CD50 antibody

Separation of leukocytes stained using anti-human CD50 (MEM-04) purified antibody (concentration in sample 3 ug/ml, GAM APC, red-filled) from leukocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Mouse Anti-CD50 Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800967

Flow Cytometry (FC/FACS) of anti-CD50 antibody

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD50 (MEM-04) purified antibody (concentration in sample 3 ug/ml, GAM APC).
Mouse Anti-CD50 Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1800967

Other Notes

Small volumes of anti-CD50 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD50 purchase

MBS1800967 is an antibody targeting CD50. Also known as Anti-Hu CD50 Purified Low Endotoxin. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD50. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
intercellular adhesion molecule 3 isoform 1
NCBI Official Synonym Full Names
intercellular adhesion molecule 3
NCBI Official Symbol
ICAM3
NCBI Official Synonym Symbols
CD50; CDW50; ICAM-R
UniProt Protein Name
Intercellular adhesion molecule 3
UniProt Synonym Protein Names
CDw50; ICAM-R
UniProt Gene Name
ICAM3
UniProt Synonym Gene Names
ICAM-3
UniProt Entry Name
ICAM3_HUMAN
NCBI Protein Information
intercellular adhesion molecule 3

NCBI Summary for CD50

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]
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UniProt Comments for CD50

ICAM3: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. Belongs to the immunoglobulin superfamily. ICAM family.

Protein type: Cell adhesion; Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: integrin binding; protein binding; receptor binding

Biological Process: cell-cell adhesion; extracellular matrix organization and biogenesis; regulation of immune response; cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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