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CD18 Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1801033

CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most… More details

$315 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD18, Antibody
Full Product Name
Anti-Hu CD18 Purified
Product Gene Name
anti-CD18 antibody
Product Synonym Gene Name
ITGB2
Product Synonym Names
integrin subunit beta 2; Integrin beta2; Complement receptor C3 subunit beta; ITGB2
Isotype
IgG1
Clone Number
MEM-148
Host
Mouse
Species Reactivity
Human
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Purity/Purification
Purified by Protein-A Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
The antibody MEM-148 recognizes an extracellular epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit; beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Preparation and Storage
Store at 2-8 degree C.
Matching Pairs
Unconjugated Antibody: CD18 Clone #MEM-148 (MBS1801033)
Unconjugated Low Endotoxin Antibody: CD18 Clone #MEM-148 (MBS1801034)
Matching Pairs
Unconjugated Antibody: CD18 Clone #MEM-148 (MBS1801033)
APC Conjugated Antibody: CD18 Clone #MEM-148 (MBS1801035)
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OMIM
116920
3D Structure
ModBase 3D Structure for P05107
Immunogen
Peripheral blood mononuclear cells
Negative Species
Pig
Applications Tested/Suitable for anti-CD18 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-CD18 antibody
FC/FACS: QC tested

Western Blot (WB) of anti-CD18 antibody

Western blotting analysis of PMA-activated neutrophils (A) and monocytes (B), using anti-human CD18 (MEM-148).
The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).
Mouse Anti-CD18 Antibody – Cat# MBS1801033

Flow Cytometry (FC/FACS) of anti-CD18 antibody

Separation of human monocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-148) purified antibody (concentration in sample 1.67 ug/ml) GAM APC.
Mouse Anti-CD18 Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1801033

Flow Cytometry (FC/FACS) of anti-CD18 antibody

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-148) purified antibody (concentration in sample 1.67 ug/ml) GAM APC.
Mouse Anti-CD18 Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1801033

Other Notes

Small volumes of anti-CD18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD18 purchase

MBS1801033 is an antibody targeting CD18. Also known as Anti-Hu CD18 Purified. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD18. It may not necessarily be applicable to this product.

NCBI GI #
89191865
NCBI Accession #
NP_000202.2
UniProt Primary Accession #
P05107
GenBank Nucleotide #
NP_000202.2
NCBI Official Full Name
integrin beta-2
UniProt Protein Name
Integrin beta-2
UniProt Synonym Protein Names
Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta
Protein Family
CD180 antigen
UniProt Gene Name
ITGB2
UniProt Synonym Gene Names
CD18; MFI7
UniProt Entry Name
ITB2_HUMAN

UniProt Comments for CD18

ITGB2: the integrin beta 2 subunit. Can combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Participates in cell adhesion as well as cell-surface mediated signaling. Defects are the cause of leukocyte adhesion deficiency type I (LAD1).

Protein type: Receptor, misc.; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis; Cell surface

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cell surface; membrane; plasma membrane; integrin complex; receptor complex; vesicle; lipid raft

Molecular Function: protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; cell adhesion molecule binding; ICAM-3 receptor activity; glycoprotein binding; protein kinase binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; apoptosis; cell-matrix adhesion; natural killer cell activation; receptor clustering; activation of NF-kappaB transcription factor; regulation of cell shape; leukocyte adhesion; cellular extravasation; cell-cell signaling; inflammatory response; cell adhesion; regulation of peptidyl-tyrosine phosphorylation; toll-like receptor 4 signaling pathway; aging; integrin-mediated signaling pathway; neutrophil chemotaxis; regulation of immune response; activated T cell proliferation; leukocyte migration during inflammatory response; heterotypic cell-cell adhesion; positive regulation of angiogenesis; toll-like receptor signaling pathway; endothelial cell migration; innate immune response; blood coagulation; leukocyte migration

Disease: Leukocyte Adhesion Deficiency, Type I
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD18 antibody

 Reference Product  PubMed Publications
 ITGAM antibody  >1599 publications with CD18 and ITGAM
 ICAM1 antibody  >858 publications with CD18 and ICAM1
 ITGAX antibody  >395 publications with CD18 and ITGAX
 CD14 antibody  >304 publications with CD18 and CD14
 ICAM2 antibody  >58 publications with CD18 and ICAM2
 ICAM3 antibody  >48 publications with CD18 and ICAM3
 SYK antibody  >38 publications with CD18 and SYK
 PRTN3 antibody  >12 publications with CD18 and PRTN3
 ITGAD antibody  >12 publications with CD18 and ITGAD
 PTK2 antibody  >9 publications with CD18 and PTK2

Pathways associated with anti-CD18 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 CXCR3-mediated Signaling Events Pathway antibodies  CXCR3-mediated Signaling Events Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with anti-CD18 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >870 publications with CD18 and Inflammation
 Necrosis Antibodies  >602 publications with CD18 and Necrosis
 Neoplasms Antibodies  >403 publications with CD18 and Neoplasms
 Cardiovascular Diseases Antibodies  >392 publications with CD18 and Cardiovascular Diseases
 Lung Diseases Antibodies  >207 publications with CD18 and Lung Diseases
 Leukocyte-Adhesion Deficiency Syndrome Antibodies  >160 publications with CD18 and Leukocyte-Adhesion Deficiency Syndrome
 Heart Diseases Antibodies  >127 publications with CD18 and Heart Diseases
 Leukocyte Disorders Antibodies  >113 publications with CD18 and Leukocyte Disorders
 Hypersensitivity Antibodies  >107 publications with CD18 and Hypersensitivity
 Drug Toxicity Antibodies  >91 publications with CD18 and Drug Toxicity

Organs/Tissues associated with anti-CD18 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3633 publications with CD18 and Blood
 Lung Antibodies  >359 publications with CD18 and Lung
 Bone Antibodies  >283 publications with CD18 and Bone
 Bone Marrow Antibodies  >254 publications with CD18 and Bone Marrow
 Heart Antibodies  >178 publications with CD18 and Heart
 Muscle Antibodies  >153 publications with CD18 and Muscle
 Liver Antibodies  >124 publications with CD18 and Liver
 Embryonic Tissue Antibodies  >121 publications with CD18 and Embryonic Tissue
 Kidney Antibodies  >101 publications with CD18 and Kidney
 Spleen Antibodies  >100 publications with CD18 and Spleen
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