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CD177 Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1801064

CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31… More details

$315 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD177, Antibody
Full Product Name
Anti-Hu CD177 Purified
Product Gene Name
Product Synonym Names
CD177 molecule; NB1; PRV1; HNA2A
Isotype
IgG1
Clone Number
MEM-166
Host
Mouse
Species Reactivity
Non-human primates, Human
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Purity/Purification
Purified by Protein-A Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
The antibody MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.
Preparation and Storage
Store at 2-8 degree C.
Matching Pairs
Unconjugated Antibody: CD177 Clone #MEM-166 (MBS1801064)
APC Conjugated Antibody: CD177 Clone #MEM-166 (MBS1801065)
Matching Pairs
Unconjugated Antibody: CD177 Clone #MEM-166 (MBS1801064)
FITC Conjugated Antibody: CD177 Clone #MEM-166 (MBS1801066)
OMIM
162860
3D Structure
ModBase 3D Structure for Q8N6Q3
Immunogen
Human granulocytes
Applications Tested/Suitable for anti-CD177 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-CD177 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-CD177 antibody

Flow cytometry (surface staining) of human peripheral blood cells with anti-CD177 (MEM-166) purified, GAM-APC.
Mouse Anti-CD177 Antibody – Cat# MBS1801064

Other Notes

Small volumes of anti-CD177 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD177 purchase

MBS1801064 is an antibody targeting CD177. Also known as Anti-Hu CD177 Purified. Reactive in Non-human primates, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD177. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD177 antigen
NCBI Official Synonym Full Names
CD177 molecule
NCBI Official Symbol
CD177
NCBI Official Synonym Symbols
NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1 GP
UniProt Protein Name
CD177 antigen
UniProt Synonym Protein Names
Human neutrophil alloantigen 2a; HNA-2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1; CD_antigen: CD177
Protein Family
UniProt Gene Name
CD177
UniProt Synonym Gene Names
NB1; PRV1; HNA-2a; NB1 GP; PRV-1
UniProt Entry Name
CD177_HUMAN
NCBI Protein Information
CD177 antigen

NCBI Summary for CD177

This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified. [provided by RefSeq, Apr 2014]
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UniProt Comments for CD177

CD177: Induced by CSF3 in resting granulocytes. Induced in patients with polycythemia vera (PV) and with essential thrombocythemia (ET). 3 isoforms of the human protein are produced by alternative splicing

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: plasma membrane

Biological Process: blood coagulation; leukocyte migration
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD177 antibody

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