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CD112 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1801503

CD112, also known as nectin-2, is a transmembrane glycoprotein involved in organization of adherens junctions. It also serves as a target molecule for entry of certain strains of herpes simplex virus (HSV) and pseudorabies virus (PRV). It is homologous to… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD112, Monoclonal Antibody
Full Product Name
Anti-Hu CD112 PE
Product Gene Name
Product Synonym Gene Name
NECTIN2
Product Synonym Names
nectin cell adhesion molecule 2; NECTIN2; HVEB; PRR2; PVRL2; PVRR2
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
R2.525
Host
Mouse
Species Reactivity
Human
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Purity/Purification
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Specificity
The mouse monoclonal antibody R2.525 recognizes an extracellular epitope on CD112, a type I transmembrane glycoprotein expressed by myelomonocytic and megakaryocytic cells, and by CD34+ hematopoietic progenitors.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Matching Pairs
Unconjugated Antibody: CD112 Clone #R2.525 (MBS1801502)
PE Conjugated Antibody: CD112 Clone #R2.525 (MBS1801503)
OMIM
X80038 mRNA
3D Structure
ModBase 3D Structure for Q92692
Immunogen
NIH/3T3 cells transfected with human Nectin-2
Conjugation
PE
Applications Tested/Suitable for anti-CD112 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD112 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-CD112 antibody

Separation of CACO-2 cells stained using anti-human CD112 (R2.525) PE antibody (10 ul reagent per million cells in 100 ul of cell suspension, red-filled) from CACO-2 cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 ug/ml, same as CD112 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of CACO-2 cell suspension.
Mouse Anti-CD112 Monoclonal Antibody – Cat# MBS1801503

Other Notes

Small volumes of anti-CD112 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD112 purchase

MBS1801503 is an antibody targeting CD112. Also known as Anti-Hu CD112 PE. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD112. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Nectin-2
NCBI Official Synonym Full Names
nectin cell adhesion molecule 2
NCBI Official Symbol
NECTIN2
NCBI Official Synonym Symbols
HVEB; PRR2; CD112; PVRL2; PVRR2
UniProt Protein Name
Nectin-2
UniProt Synonym Protein Names
Herpes virus entry mediator B; Herpesvirus entry mediator B; HveB; Nectin cell adhesion molecule 2
Protein Family
UniProt Gene Name
NECTIN2
UniProt Synonym Gene Names
Herpesvirus entry mediator B; HveB
NCBI Protein Information
nectin-2

NCBI Summary for CD112

This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD112

Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive (PubMed:26755705). Probable cell adhesion protein (PubMed:9657005).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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