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SIT Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1801529

SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal… More details

$330 / 0.1 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SIT, Antibody
Full Product Name
Anti-SIT Purified
Product Gene Name
Product Synonym Gene Name
SIT1
Product Synonym Names
signaling threshold regulating transmembrane adapt; SIT1
Isotype
IgG1
Clone Number
SIT-01
Host
Mouse
Species Reactivity
Human
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Purity/Purification
Purified by Protein-A Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT.
Preparation and Storage
Store at 2-8 degree C.
Matching Pairs
Unconjugated Antibody: SIT Clone #SIT-01 (MBS1801529)
PE Conjugated Antibody: SIT Clone #SIT-01 (MBS1801530)
OMIM
604964
3D Structure
ModBase 3D Structure for Q9Y3P8
Immunogen
Bacterially produced recombinant intracellular fragment of human SIT.
Applications Tested/Suitable for anti-SIT antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)

Western Blot (WB) of anti-SIT antibody

Western blotting analysis of human SIT using mouse monoclonal antibody SIT-01 on lysates of Molt-4 and HEK-293T cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 ug/ml of mouse anti-SIT monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. SIT was detected around 36 kDa.
Mouse Anti-SIT Antibody – Cat# MBS1801529

Flow Cytometry (FC/FACS) of anti-SIT antibody

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-SIT (SIT-01) purified antibody (concentration in sample 9 ug/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-SIT Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1801529

Flow Cytometry (FC/FACS) of anti-SIT antibody

Separation of human CD3 negative SIT positive lymphocytes (red-filled) from CD3 negative SIT negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-SIT (SIT-01) purified antibody (concentration in sample 9 ug/ml, GAM APC).
Mouse Anti-SIT Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1801529

Flow Cytometry (FC/FACS) of anti-SIT antibody

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-SIT (SIT-01) purified antibody (concentration in sample 9 ug/ml, GAM APC).
Mouse Anti-SIT Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1801529

Other Notes

Small volumes of anti-SIT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SIT purchase

MBS1801529 is an antibody targeting SIT. Also known as Anti-SIT Purified. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SIT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
signaling threshold-regulating transmembrane adapter 1
NCBI Official Synonym Full Names
signaling threshold regulating transmembrane adaptor 1
NCBI Official Symbol
SIT1
NCBI Official Synonym Symbols
SIT; SIT-R
UniProt Protein Name
Signaling threshold-regulating transmembrane adapter 1
UniProt Synonym Protein Names
SHP2-interacting transmembrane adapter protein; Suppression-inducing transmembrane adapter 1; gp30/40
UniProt Gene Name
SIT1
UniProt Synonym Gene Names
SIT
UniProt Entry Name
SIT1_HUMAN
NCBI Protein Information
signaling threshold-regulating transmembrane adapter 1

UniProt Comments for SIT

SIT: a low molecular weight transmembrane adaptor protein. A disulfide-linked homodimer. Expressed in lymphocytes. Following phosphorylation by Src and possibly Tec kinases, recruits the SH2 domain-containing tyrosine phosphatase SHP2 via an immunoreceptor tyrosine-based inhibition motif (ITIM).

Protein type: Membrane protein, integral; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9p13-p12

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; SH2 domain binding; kinase binding

Biological Process: immune system process; signal transduction; regulation of T cell activation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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