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TPX2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1801591

TPX2 is a microtubule-associated protein, which is a critical regulator of mitosis. At the beginning of mitosis, TPX2 is released and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division.… More details

$340 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
TPX2, Monoclonal Antibody
Full Product Name
Anti-TPX2 Purified
Product Gene Name
Product Synonym Names
TPX2 microtubule nucleation factor; DIL2; HCTP4; REPP86
Clonality
Monoclonal
Isotype
IgG1
Clone Number
TPX2-01
Host
Mouse
Species Reactivity
Rat, Mouse, Human
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Purity/Purification
Purified by Protein-A Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
The mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated intracellular critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.
Preparation and Storage
Store at 2-8 degree C.
OMIM
605917
3D Structure
ModBase 3D Structure for Q9ULW0
Immunogen
Human TPX2
Applications Tested/Suitable for anti-TPX2 antibody
Immunoprecipitation, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), ELISA (EIA)

Western Blot (WB) of anti-TPX2 antibody

Western blotting analysis of TPX2 using monoclonal antibody TPX2-01 in A) human cell lines, B) murine (P19, NIH 3T3, Neuro-2a) and rat (RBL-2H3, L6) cell lines.
Mouse Anti-TPX2 Monoclonal Antibody – Cat# MBS1801591

Immunoprecipitation (IP) of anti-TPX2 antibody

Immunoprecipitation of TPX2 from nuclear extract of HEK293 cells using monoclonal antibody TPX2-01. 1) nuclear extract, 2) antibody, 3) immunoprecipitate, 4) carrier
Mouse Anti-TPX2 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS1801591

Immunocytochemistry (ICC) of anti-TPX2 antibody

Immunocytochemistry of TPX2 using monoclonal antibody TPX2-01 in glioblastoma cell line T98G: a) interphase, b) mitosis. c) Immunohistochemistry of TPX2 in U87MG human glioblastoma cells (orthotopic xenograft in murine brain). Bar: 20 um.
Mouse Anti-TPX2 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS1801591

Other Notes

Small volumes of anti-TPX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPX2 purchase

MBS1801591 is an antibody targeting TPX2. Also known as Anti-TPX2 Purified. Reactive in Rat, Mouse, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TPX2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
targeting protein for Xklp2
NCBI Official Synonym Full Names
TPX2 microtubule nucleation factor
NCBI Official Symbol
TPX2
NCBI Official Synonym Symbols
DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
UniProt Protein Name
Targeting protein for Xklp2
UniProt Synonym Protein Names
Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Protein fls353; Restricted expression proliferation-associated protein 100
Protein Family
UniProt Gene Name
TPX2
UniProt Synonym Gene Names
C20orf1; C20orf2; DIL2; HCA519; DIL-2; p100
UniProt Entry Name
TPX2_HUMAN
NCBI Protein Information
targeting protein for Xklp2

UniProt Comments for TPX2

TPX2: a microtubule-associated protein required for meiotic and mitotic spindle assembly. Targets a plus end-directed motor (XKLP2) to the minus ends of microtubules in the mitotic spindle. Required for microtubule nucleation around chromosomes in response to the local generation of RanGTP. Binds to and activates Aurora A kinase and targets it to the mitotic spindle.

Protein type: Microtubule-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: nucleoplasm; microtubule cytoskeleton; axon hillock; spindle pole; microtubule; cytoplasm; spindle; nucleus

Molecular Function: protein binding; GTP binding; protein kinase binding; ATP binding

Biological Process: mitosis; cell proliferation; apoptosis; cell division; activation of protein kinase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TPX2 antibody

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