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Gamma-Tubulin (TUBG1) Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1801632

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation… More details

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Product Overview

Online Datasheet in PDF
Product Name
gamma-Tubulin (TUBG1), Antibody
Full Product Name
Anti-gamma-Tubulin Purified
Product Gene Name
anti-TUBG1 antibody
Product Synonym Names
tubulin gamma 1; TUBG
Isotype
IgG1
Clone Number
TU-32
Host
Mouse
Species Reactivity
Protozoa, Plants, Animals
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Purity/Purification
Purified by Protein-A Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
The antibody TU-32 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
Preparation and Storage
Store at 2-8 degree C.
OMIM
191135
3D Structure
ModBase 3D Structure for P23258
Immunogen
human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449
Applications Tested/Suitable for anti-TUBG1 antibody
Western Blot (WB), Immunocytochemistry (ICC)

Western Blot (WB) of anti-TUBG1 antibody

Western blotting analysis of differential reactivity of monoclonal antibodies to ?-tubulin with human ?-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human ?-tubulin 1 (?-Tb1) or ?-tubulin 2 (?-Tb2), probed with Abs to ?-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of ?-Tb1 or ?-Tb2 probed with Abs to ?-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.
Mouse Anti-TUBG1 Antibody – Cat# MBS1801632

Western Blot (WB) of anti-TUBG1 antibody

Western blotting analysis of human gamma-tubulin using mouse monoclonal antibody TU-32 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 ug/ml of mouse anti-gamma-tubulin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for gamma-tubulin at approximately 46 kDa.
Mouse Anti-TUBG1 Antibody, Western Blot (WB) – Cat# MBS1801632

Other Notes

Small volumes of anti-TUBG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TUBG1 purchase

MBS1801632 is an antibody targeting TUBG1. Also known as Anti-gamma-Tubulin Purified. Reactive in Protozoa, Plants, Animals. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TUBG1. It may not necessarily be applicable to this product.

NCBI GI #
31543831
NCBI GeneID
7283
NCBI Accession #
NP_001061.2
UniProt Primary Accession #
P23258
UniProt Related Accession #
P23258
GenBank Nucleotide #
NP_001061.2
NCBI Official Full Name
tubulin gamma-1 chain
NCBI Official Synonym Full Names
tubulin gamma 1
NCBI Official Symbol
TUBG1
NCBI Official Synonym Symbols
TUBG; GCP-1; CDCBM4; TUBGCP1
UniProt Protein Name
Tubulin gamma-1 chain
UniProt Synonym Protein Names
Gamma-1-tubulin; Gamma-tubulin complex component 1
Protein Family
Gamma-tubulin complex
UniProt Gene Name
TUBG1
UniProt Synonym Gene Names
TUBG; GCP-1
UniProt Entry Name
TBG1_HUMAN
NCBI Protein Information
tubulin gamma-1 chain

NCBI Summary for TUBG1

This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]
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UniProt Comments for TUBG1

TUBG1: Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO/C1orf88. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: centriole; centrosome; recycling endosome; pericentriolar material; condensed nuclear chromosome; cytoplasmic microtubule; nonmotile primary cilium; apical part of cell; cytoplasm; leading edge; polar microtubule; cytosol; gamma-tubulin complex

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: metabolic process; organelle organization and biogenesis; mitotic cell cycle; microtubule cytoskeleton organization and biogenesis; microtubule nucleation; G2/M transition of mitotic cell cycle; cytoplasmic microtubule organization and biogenesis; meiotic spindle organization and biogenesis

Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 4
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Research Articles on TUBG1

1. TACC3 phosphorylation stabilizes gamma- tubulin ring complex assembly and thereby regulates formation of centrosomal asters.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TUBG1 antibody

 Reference Product  PubMed Publications
 PCNT antibody  >1 publications with TUBG1 and PCNT

Pathways associated with anti-TUBG1 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Centrosome Maturation Pathway antibodies  Centrosome Maturation Pathway Diagram
 G2/M Transition Pathway antibodies  G2/M Transition Pathway Diagram
 Loss Of Nlp From Mitotic Centrosomes Pathway antibodies  Loss Of Nlp From Mitotic Centrosomes Pathway Diagram
 Loss Of Proteins Required For Interphase Microtubule Organization From The Centrosome Pathway antibodies  Loss Of Proteins Required For Interphase Microtubule Organization From The Centrosome Pathway Diagram
 Mitotic G2-G2/M Phases Pathway antibodies  Mitotic G2-G2/M Phases Pathway Diagram
 PLK1 Signaling Events Pathway antibodies  PLK1 Signaling Events Pathway Diagram
 Recruitment Of NuMA To Mitotic Centrosomes Pathway antibodies  Recruitment Of NuMA To Mitotic Centrosomes Pathway Diagram
 Recruitment Of Mitotic Centrosome Proteins And Complexes Pathway antibodies  Recruitment Of Mitotic Centrosome Proteins And Complexes Pathway Diagram

Diseases associated with anti-TUBG1 antibody

 Disease Name  Pubmed Publications
 Drug Toxicity Antibodies  >1 publications with TUBG1 and Drug Toxicity
 Carcinoma Antibodies  >1 publications with TUBG1 and Carcinoma
 Multiple Myeloma Antibodies  >1 publications with TUBG1 and Multiple Myeloma
 Nervous System Diseases Antibodies  >1 publications with TUBG1 and Nervous System Diseases
 Breast Neoplasms Antibodies  >1 publications with TUBG1 and Breast Neoplasms
 Microcephaly Antibodies  >1 publications with TUBG1 and Microcephaly
 Malformations of Cortical Development Antibodies  >1 publications with TUBG1 and Malformations of Cortical Development

Organs/Tissues associated with anti-TUBG1 antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >3 publications with TUBG1 and Embryonic Tissue
 Brain Antibodies  >2 publications with TUBG1 and Brain
 Muscle Antibodies  >1 publications with TUBG1 and Muscle
 Lung Antibodies  >1 publications with TUBG1 and Lung
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