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Class I (HLA) Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1801703

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by… More details

$430 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HLA Class I, Antibody
Full Product Name
Anti-HLA Class I Biotin
Isotype
IgG2a
Clone Number
W6/32
Host
Mouse
Species Reactivity
Cow, Non-human primates, Human, Cat
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Purity/Purification
The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Concentration
1 mg/ml (lot specific)
Specificity
The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.
Preparation and Storage
Store at 2-8 degree C.
Matching Pairs
Unconjugated Antibody: HLA Class I Clone #W6/32 (MBS1801701)
Biotin Conjugated Antibody: HLA Class I Clone #W6/32 (MBS1801703)
Immunogen
Membrane of human tonsil cells
Negative Species
Rabbit
Conjugation
Biotin
Applications Tested/Suitable for anti-HLA Class I antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry (IHC) Frozen, Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-HLA Class I antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-HLA Class I antibody

Separation of human leukocytes (red-filled) from HLA Class I negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human HLA Class I (W6/32) Biotin antibody (concentration in sample 4 ug/ml, Streptavidin APC).
Mouse Anti-HLA Antibody – Cat# MBS1801703

Flow Cytometry (FC/FACS) of anti-HLA Class I antibody

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA Class I (W6/32) Biotin antibody (concentration in sample 4 ug/ml, Streptavidin APC).
Mouse Anti-HLA Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1801703

Other Notes

Small volumes of anti-HLA Class I antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HLA Class I purchase

MBS1801703 is an antibody targeting HLA Class I. Also known as Anti-HLA Class I Biotin. Reactive in Cow, Non-human primates, Human, Cat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HLA Class I. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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