Human 5-NT Recombinant Protein – Cat# MBS184015

Ecto-5-Nucleotidase (5-NT) Human Recombinant Protein

Name: 5-NT Recombinant Protein; Ecto-5-Nucleotidase; Human; MyBioSource
Brand: MyBioSource
SKU: MBS184015
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS184015

This protein is a dimeric enzyme that is expressed on the exterior face of the plasmamembrane (1, 2). CD73 acts by hydrolysing nucleotide monophosphates, there by releasingin organic phosphate and the corresponding nucleoside. Exhibits AMP-, NAD-, and NMN… More details

$545 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Ecto-5-nucleotidase (5-NT), Recombinant Protein

Full Product Name

Recombinant Human Ecto-5-nucleotidase, 5-NT or CD73

Product Gene Name

5-NT recombinant protein

Product Synonym Gene Name

CD73

Product Synonym Names

5-Nucleotidase (5-NT); Cluster of Differentiation 73 (CD73)

Sequence

WELTILHTND VHSRLEQTSE DSSKCVNASR CMGGVARLFT KVQQIRRAEP NVLLLDAGDQ YQGTIWFTVY KGAEVAHFMN ALRYDAMALG NHEFDNGVEG LIEPLLKEAK FPILSANIKA KGPLASQISG LYLPYKVLPV GDEVVGIVGY TSKETPFLSN PGTNLVFEDE ITALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DVVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTSDDGRKVP VVQAYAFGKY LGYLKIEFDE RGNVISSHGN PILLNSSIPE DPSIKADINK WRIKLDNYST QELGKTIVYL DGSSQSCRFR ECNMGNLICD AMINNNLRHT DEMFWNHVSM CILNGGGIRS PIDERNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDLSRKPG DRVVKLDVLC TKCRVPSYDP LKMDEVYKVI LPNFLANGGD GFQMIKDELL RHDSGDQDIN VVSTYISKMK VIYPAVEGRI KFSHHHHHH

Host

HEK293 Cells

Form/Format

Liquid. PBS 20% Glycerol

Purity/Purification

Affinity chromatography. >95% as estimated by SDS-PAGE stained with Instant Blue Stain (Expedeon).

Preparation and Storage

Store at -20 degree or -70 degree C for at least 12 months
Shipping: Dry ice or frozen gel packs

Molecular Weight

574

Sequence Positions

27-549aa

OMIM

gene 211800

3D Structure

ModBase 3D Structure for P21589

Source

Human

Tag

6xHis at the C-terminus

Endotoxin

Less than 0.1ng/ug (1 IEU/ug), as measured by LAL method.

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Applications Tested/Suitable for 5-NT recombinant protein

Western Blot (WB), Immunoassay (IA), Functional Studies

Product References and Citations for 5-NT recombinant protein

Heuts DPHM., Weissenborn MJ., Olkhov RV., Shaw AM., Gummadova JO., Levy C. and NS. Scrutton. Crystal Structure of a Soluble Form of Human CD73 with Ecto-5'-NucleotidaseActivity. ChemBioChem. Vol. 13, No 16, pp 2384-2391. 2012.

Other Notes

Small volumes of 5-NT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 5-NT purchase

MBS184015 is a recombinant protein targeting 5-NT. Also known as Recombinant Human Ecto-5-nucleotidase, 5-NT or CD73. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for 5-NT. It may not necessarily be applicable to this product.

NCBI GI #

112825

NCBI GeneID

4907

NCBI Accession #

P21589.1

UniProt Primary Accession #

P21589

UniProt Secondary Accession #

O75520; Q5W116; B3KQI8

UniProt Related Accession #

P21589

NCBI Official Full Name

5'-nucleotidase

NCBI Official Synonym Full Names

5'-nucleotidase, ecto (CD73)

NCBI Official Symbol

NT5E

NCBI Official Synonym Symbols

NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA

UniProt Protein Name

5'-nucleotidase

UniProt Synonym Protein Names

Ecto-5'-nucleotidase; CD_antigen: CD73

Protein Family

Snake venom 5'-nucleotidase

UniProt Gene Name

NT5E

UniProt Synonym Gene Names

NT5; NTE; 5'-NT

UniProt Entry Name

5NTD_HUMAN

NCBI Protein Information

5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase

NCBI Summary for 5-NT

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

UniProt Comments for 5-NT

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Phosphatase (non-protein); Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries

Research Articles on 5-NT

1. we report that ATP-mediated suppression of IFNgamma production by Th17 cells can be overcome by genetic ablation of CD73 or by using IL1beta instead of TGFbeta to program Th17 cells ex vivo

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with 5-NT recombinant protein

Reference Product	PubMed Publications
ENPP1 recombinant protein	>4 publications with 5-NT and ENPP1
ADA recombinant protein	>2 publications with 5-NT and ADA
ENTPD1 recombinant protein	>2 publications with 5-NT and ENTPD1

Pathways associated with 5-NT recombinant protein

Products by Pathway	Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nucleotides Pathway antibodies	Metabolism Of Nucleotides Pathway Diagram
Nicotinate And Nicotinamide Metabolism Pathway antibodies	Nicotinate And Nicotinamide Metabolism Pathway Diagram
Nicotinate And Nicotinamide Metabolism Pathway antibodies	Nicotinate And Nicotinamide Metabolism Pathway Diagram
Purine Catabolism Pathway antibodies	Purine Catabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram

Diseases associated with 5-NT recombinant protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>17 publications with 5-NT and Inflammation
Necrosis Antibodies	>9 publications with 5-NT and Necrosis
Breast Neoplasms Antibodies	>9 publications with 5-NT and Breast Neoplasms
Nervous System Diseases Antibodies	>9 publications with 5-NT and Nervous System Diseases
Disease Models, Animal Antibodies	>8 publications with 5-NT and Disease Models, Animal
Pain Antibodies	>7 publications with 5-NT and Pain
Brain Diseases Antibodies	>5 publications with 5-NT and Brain Diseases
Kidney Diseases Antibodies	>4 publications with 5-NT and Kidney Diseases
Heart Diseases Antibodies	>4 publications with 5-NT and Heart Diseases
Acute Kidney Injury Antibodies	>3 publications with 5-NT and Acute Kidney Injury

Organs/Tissues associated with 5-NT recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>33 publications with 5-NT and Blood
Vascular Antibodies	>20 publications with 5-NT and Vascular
Bone Antibodies	>13 publications with 5-NT and Bone
Brain Antibodies	>9 publications with 5-NT and Brain
Bone Marrow Antibodies	>8 publications with 5-NT and Bone Marrow
Muscle Antibodies	>8 publications with 5-NT and Muscle
Mouth Antibodies	>8 publications with 5-NT and Mouth
Kidney Antibodies	>6 publications with 5-NT and Kidney
Ganglia Antibodies	>6 publications with 5-NT and Ganglia
Embryonic Tissue Antibodies	>5 publications with 5-NT and Embryonic Tissue