Beta Defensins (BDF) ELISA Kit for Bovine

Name: Bovine BDF ELISA Kit; Beta Defensins; MyBioSource
Brand: MyBioSource
SKU: MBS018536
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS018536

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of BDF (hereafter termed "analyte") in undiluted original Bovine body fluids, tissue homogenates, secretions… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

beta Defensins (BDF), ELISA Kit

Full Product Name

Bovine beta Defensins ELISA Kit

Product Gene Name

BDF elisa kit

Species Reactivity

Bovine

Assay Type

Quantitative Sandwich

Samples

undiluted original Bovine body fluids, tissue homogenates, secretions or feces samples

Detection Range

31.2pg/ml-1000pg/mL

Sensitivity

5.0pg/mL

Specificity

No significant cross-reactivity or interference between this analyte and analogues is observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

7,697 Da

OMIM

606611

3D Structure

ModBase 3D Structure for P81534

Other Notes

Small volumes of BDF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BDF purchase

MBS018536 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the beta Defensins (BDF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BDF. The ELISA analytical biochemical technique of the MBS018536 kit is based on BDF antibody-BDF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BDF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BDF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for BDF. It may not necessarily be applicable to this product.

NCBI GI #

8923890

NCBI GeneID

55894

NCBI Accession #

NP_061131.1

UniProt Primary Accession #

P81534

UniProt Secondary Accession #

Q8NFG6; Q9NPF6

UniProt Related Accession #

P81534

GenBank Nucleotide #

NM_018661.4

NCBI Official Full Name

beta-defensin 103

NCBI Official Synonym Full Names

defensin, beta 103B

NCBI Official Symbol

DEFB103B

NCBI Official Synonym Symbols

BD-3; HBD3; HBP3; DEFB3; HBD-3; HBP-3; DEFB-3; DEFB103

UniProt Protein Name

Beta-defensin 103

UniProt Synonym Protein Names

Beta-defensin 3; BD-3; DEFB-3; HBD3; hBD-3; Defensin, beta 103; Defensin-like protein

UniProt Gene Name

DEFB103A

UniProt Synonym Gene Names

BD3; DEFB103; DEFB3; BD-3; DEFB-3; HBD3; hBD-3

UniProt Entry Name

D103A_HUMAN

NCBI Protein Information

beta-defensin 103; beta-defensin 3; defensin, beta 103; defensin-like protein

NCBI Summary for BDF

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103B, which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. [provided by RefSeq, Jul 2008]

UniProt Comments for BDF

defensin, beta 3: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed. Belongs to the beta-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: Golgi lumen; extracellular region

Biological Process: defense response to bacterium; innate immune response; positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria

Research Articles on BDF

1. Data show that Lactobacillus crispatus promotes epithelial cell defense against Candida albicans infection through the involvement of TLR2/4, IL-8 and human beta-defensin 2 and 3, suggesting this Lactobacillus as an anti-infective agent against C. albicans.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with BDF elisa kit

Products by Pathway	Pathway Diagram
Beta Defensins Pathway antibodies	Beta Defensins Pathway Diagram
Defensins Pathway antibodies	Defensins Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram