Fen-1 (FEN1) Antibody – Mouse Monoclonal

FEN1: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11. Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Protein type: EC 3.1.-.-; Ribonuclease; DNA repair, damage; DNA-binding; Nucleolus; Nuclear receptor co-regulator; Deoxyribonuclease

Cellular Component: mitochondrion; membrane; nucleolus; plasma membrane; nucleus

Molecular Function: nuclease activity; hydrolase activity; 5'-flap endonuclease activity; metal ion binding; magnesium ion binding; hydrolase activity, acting on ester bonds; T/G mismatch-specific endonuclease activity; flap endonuclease activity; phosphoric ester hydrolase activity; 5'-3' exonuclease activity; DNA binding; manganese ion binding; endonuclease activity; exonuclease activity; ribonuclease H activity; catalytic activity

Biological Process: DNA replication, removal of RNA primer; DNA replication; DNA repair; response to DNA damage stimulus; DNA catabolic process, endonucleolytic; memory