Mouse Anti-CAST Monoclonal Antibody – Cat# MBS190221

Human Calpastatin (CAST) Antibody – Mouse Monoclonal

Name: CAST Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS190221
Price: 625 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS190221

MBS190221 is an antibody targeting CAST. Also known as Human Calpastatin Monoclonal Antibody. Host: Mouse. More details

$625 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Human Calpastatin (CAST), Monoclonal Antibody

Full Product Name

Human Calpastatin Monoclonal Antibody

Product Gene Name

anti-CAST antibody

Product Synonym Names

Human Calpastatin; Calpastatin

Clonality

Monoclonal

Host

Mouse

Form/Format

100 ug IgG in 10 mM PBS, pH 7.4, lyophilized.

Specificity

Human Calpastatin [Mouse monoclonal antibody to human Calpastatin]
This antibody specifically reacts with human calpastatin
Epitope: Domain III
Sub-class: IgG1

Preparation and Storage

Lyophilized antibody is stable at 4 degree C for 2 years. Store the reconstituted solution in aliquots at -20 degree C for one year or at 4 degree C for 6 months. Avoid repeated freeze-thaw cycles.

Molecular Weight

76,573 Da

OMIM

114090

3D Structure

ModBase 3D Structure for P20810

Antigen

Human Calpastatin

Stabilizer

1% BSA

Preservatives

0.1% Sodium Azide

Dilutions Instructions

Reconstitute in distilled water to the final desired concentration.

Product Categories/Family for anti-CAST antibody

Neuroscience and Signal Transduction Antibodies

Applications Tested/Suitable for anti-CAST antibody

ELISA, Western Blot

Other Notes

Small volumes of anti-CAST antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CAST. It may not necessarily be applicable to this product.

NCBI GI #

180975

NCBI GeneID

831

NCBI Accession #

AAA52066.1

UniProt Primary Accession #

P20810

UniProt Secondary Accession #

O95360; Q05DE8; Q7Z4K0; Q96D08; Q9H1Z5

UniProt Related Accession #

P20810; Q13940; Q15786; Q59HE3; Q6AZE3; Q7KYS9; Q7Z4K0; Q86YM9; Q9Y2M4

NCBI Official Full Name

calpastatin

NCBI Official Synonym Full Names

calpastatin

NCBI Official Symbol

CAST

NCBI Official Synonym Symbols

BS-17; MGC9402; CAST

UniProt Protein Name

Calpastatin

UniProt Synonym Protein Names

Calpain inhibitor; Sperm BS-17 component

Protein Family

Calpastatin

UniProt Gene Name

CAST

UniProt Entry Name

ICAL_HUMAN

NCBI Protein Information

calpastatin; calpain inhibitor; OTTHUMP00000158520; sperm BS-17 component; heart-type calpastatin

NCBI Summary for CAST

The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length natures of only some have been determined. [provided by RefSeq]

UniProt Comments for CAST

calpastatin: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. Belongs to the protease inhibitor I27 (calpastatin) family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein binding; endopeptidase inhibitor activity; cysteine protease inhibitor activity

Disease: Peeling Skin With Leukonychia, Acral Punctate Keratoses, Cheilitis, And Knuckle Pads

Research Articles on CAST

1. recruitment of calpastatin into aggregates allows translocation and activation of protease to the membranes; on the contrary, the presence of large amounts of calpastatin in the cytosol prevents both processes, protecting the cell from proteolysis
2. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
3. Proof of concept that the calpastatin-based reagents may be useful to selectively detect the active conformation of calpain.
4. genetically determined IL-1alpha levels may modulate transcription of calpain and calpastatin
5. Role of the calpain-calpastatin system in the density-dependent growth arrest.
6. c-Myc regulates calpain activity through calpastatin; apoptosis induced by calpain inhibition is dependent on c-Myc, and calpastatin knockdown promotes transformation in c-Myc-negative cells
7. NMR data of calapastin tripartite binding mode to capain induced by calcium were presented.
8. performed a full NMR assignment of hCSD1 to characterize it in its solution state
9. Results suggest a regulation on the calpain-calpastatin expression response to muscle damaging eccentric exercise, but not concentric exercise.
10. Genome-wide association study of gene-disease association and pharmacogenomic / toxicogenomic. (HuGE Navigator)
11. Observational study of gene-disease association. (HuGE Navigator)
12. The activity of calpain in human peripheral blood lymphocytes, was estimated by assessing the levels of limited proteolysis of calpastatin.
13. CS(L) modulates Ca2+-channel activity through interacting with the calmodulin-binding site on the C-terminal tail of the Cav1.2 channel.
14. involved in the proteolysis of amyloid precursor protein, which is thought to be abnormal in patients with Alzheimer's disease
15. calpastatin and calpain-1 represent critical proximal elements in a cascade of pro-apoptotic events leading to Bax, mitochondria, and caspase-3 activation
16. Overexpression of calpastatin reduced muscle atrophy during 10 day unloading period. Overexpression completely prevented shift in myofibrillar myosin content from slow to fast isoforms, which normally occurs in muscle unloading.
17. Overexpression of calpastatin in transgenic mice is associated with an increase in GLUT4 protein.
18. Calpastatin amino acid side chains at leucine-11 and isoleucine-18 interact with hydrophobic pockets in calpain, and each of these interactions is indispensable for effective inhibition of calpain.
19. A complete calpain-calpastatin system is expressed in the human oocyte and may play a role in the various calcium-mediated processes occurring during activation of human oocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CAST antibody

Reference Product	PubMed Publications
CAPN1 antibody	>21 publications with CAST and CAPN1
CASP3 antibody	>13 publications with CAST and CASP3
CASP1 antibody	>2 publications with CAST and CASP1
PPFIA4 antibody	>1 publications with CAST and PPFIA4
PPFIA1 antibody	>1 publications with CAST and PPFIA1
CAPNS1 antibody	>1 publications with CAST and CAPNS1

Diseases associated with anti-CAST antibody

Disease Name	Pubmed Publications
Vascular Diseases Antibodies	>871 publications with CAST and Vascular Diseases
Neoplasms Antibodies	>767 publications with CAST and Neoplasms
Death Antibodies	>454 publications with CAST and Death
Congenital Abnormalities Antibodies	>450 publications with CAST and Congenital Abnormalities
Kidney Diseases Antibodies	>424 publications with CAST and Kidney Diseases
Necrosis Antibodies	>370 publications with CAST and Necrosis
Lung Diseases Antibodies	>246 publications with CAST and Lung Diseases
Musculoskeletal Abnormalities Antibodies	>237 publications with CAST and Musculoskeletal Abnormalities
Myocardial Ischemia Antibodies	>191 publications with CAST and Myocardial Ischemia
Liver Diseases Antibodies	>138 publications with CAST and Liver Diseases

Organs/Tissues associated with anti-CAST antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>3169 publications with CAST and Bone
Blood Antibodies	>1796 publications with CAST and Blood
Mouth Antibodies	>1246 publications with CAST and Mouth
Vascular Antibodies	>1062 publications with CAST and Vascular
Muscle Antibodies	>843 publications with CAST and Muscle
Brain Antibodies	>605 publications with CAST and Brain
Skin Antibodies	>580 publications with CAST and Skin
Kidney Antibodies	>567 publications with CAST and Kidney
Heart Antibodies	>532 publications with CAST and Heart
Nerve Antibodies	>402 publications with CAST and Nerve