• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Mitosin Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS190324

MBS190324 is an antibody targeting Mitosin. Also known as Mitosin Monoclonal Antibody. Host: Mouse. More details

$620 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Mitosin, Monoclonal Antibody
Full Product Name
Mitosin Monoclonal Antibody
Product Synonym Names
Anti-Mitosin; Mitosin
Clonality
Monoclonal
Isotype
IgG2a kappa
Host
Mouse
Form/Format
100 ug Protein G purified antibody in PBS, pH 7.4
Specificity
Anti-Mitosin [Murine MAb recognizes mitosin, a 350 kDa phosphoprotein involved in mitotic-phase progression.]
This antibody recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
Preparation and Storage
This antibody is stable for at least one (1) year at -70 degree C. Avoid multiple freeze thaw cycles.
Antigen
GST fusion protein expressed inE. coli corresponding to aa 1759-2093 of human mitosin.
Stabilizer
None
Preservatives
None. Available on request.
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.

Product Categories/Family for anti-Mitosin antibody

Applications Tested/Suitable for anti-Mitosin antibody
Immunoblot, Immunohistochemistry, Immunoprecipitation
Application Notes for anti-Mitosin antibody
Immunoblotting: use at 1-10 ug/ml.
Immunoprecipitation: use at 1-10 ug/ml.
Immunohistochemistry: use at 10 ug/mlwith frozen or paraffin-embeddedsections.
Positive controls: Human tissue containingrapidly proliferating cells, such aslymphoid germinal centers.

Product References and Citations for anti-Mitosin antibody

Allred, DC et al. (1997) Lab Invest 15A(69); Zhu, X et al. (1995), Mol Cell Biol 15:5017-5029.

Other Notes

Small volumes of anti-Mitosin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Mitosin. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
mitosin
Protein Family

NCBI Summary for Mitosin

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000