• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ATG12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS191007

Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Atg13 is a target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status… More details

$580 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Atg12, Polyclonal Antibody
Full Product Name
Atg12
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Form/Format
Peptide affinity-purified antibody in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Concentration
1mg/ml (lot specific)
Specificity
This antibody recognizes human Atg12.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Molecular Weight
7,746 Da
OMIM
AB017507 mRNA
3D Structure
ModBase 3D Structure for O94817
Antigen
Synthetic peptide corresponding to amino acids at the C-terminus of human Atg12.
Applications Tested/Suitable for anti-Atg12 antibody
Immunoblotting (IB), Immunofluorescence (IF)
Application Notes for anti-Atg12 antibody
Immunoblotting: use at 1-2ug/ml. Bands of ~55kDa, corresponding to Atg12-Atg5 complex, and ~15kda, corresponding to free Atg12, are detected.
Immunofluorescence: use at 10ug/ml.
Dilute in PBS or medium that is identical to that used in the assay system.

Immunofluorescence (IF) of anti-Atg12 antibody

Rabbit Anti-ATG12 Polyclonal Antibody – Cat# MBS191007

Other Notes

Small volumes of anti-Atg12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Atg12 purchase

MBS191007 is an antibody targeting Atg12. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Atg12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin-like protein ATG12 isoform 2
NCBI Official Synonym Full Names
autophagy related 12
NCBI Official Symbol
ATG12
NCBI Official Synonym Symbols
APG12; FBR93; APG12L; HAPG12
UniProt Protein Name
Ubiquitin-like protein ATG12
UniProt Synonym Protein Names
Autophagy-related protein 12; APG12-like
Protein Family
UniProt Gene Name
ATG12
UniProt Synonym Gene Names
APG12; APG12L; APG12-like
NCBI Protein Information
ubiquitin-like protein ATG12

NCBI Summary for Atg12

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
Show more

UniProt Comments for Atg12

ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 5q22.3

Cellular Component: cytosol; phagocytic vesicle membrane; pre-autophagosomal structure membrane

Molecular Function: APG8 conjugating enzyme activity; protein binding

Biological Process: autophagic vacuole formation; C-terminal protein lipidation; macroautophagy; mitochondrion degradation
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000