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c-JUN Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS191015

MBS191015 is an antibody targeting c-Jun. Also known as c-Jun (Phospho-Ser73). Host: Rabbit. More details

$465 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
c-Jun, Polyclonal Antibody
Full Product Name
c-Jun (Phospho-Ser73)
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Form/Format
100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
Specificity
This antibody detects endogenous human, mouse, and rat c-Jun only when phosphorylated at serine 73.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Can be stored at 4 degree C for short-term.
Molecular Weight
35,676 Da
OMIM
J04111 Genomic DNA
3D Structure
ModBase 3D Structure for P05412
Antigen
Peptide sequence that includes phosphorylation site of serine 73 (L-A-S(p)-P-E) derived from human c-Jun and conjugated to KLH.
Applications Tested/Suitable for anti-c-Jun antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-c-Jun antibody
Western Blot: use at dilution of 1:500- 1:1,000. A band of ~43kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100.
Dilute in PBS or medium that is identical to that used in the assay system.

Immunohistochemistry (IHC) of anti-c-Jun antibody

Rabbit Anti-c-JUN Polyclonal Antibody – Cat# MBS191015

Testing Data of anti-c-Jun antibody

Rabbit Anti-c-JUN Polyclonal Antibody, Testing Data – Cat# MBS191015

Other Notes

Small volumes of anti-c-Jun antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for c-Jun. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription factor AP-1
NCBI Official Synonym Full Names
Jun proto-oncogene, AP-1 transcription factor subunit
NCBI Official Symbol
JUN
NCBI Official Synonym Symbols
AP1; AP-1; c-Jun
UniProt Protein Name
Transcription factor AP-1
UniProt Synonym Protein Names
Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39
UniProt Gene Name
JUN
UniProt Synonym Gene Names
AP1
NCBI Protein Information
transcription factor AP-1

NCBI Summary for c-Jun

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
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UniProt Comments for c-Jun

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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