Anti-BCL-2 Polyclonal Antibody – Cat# MBS191076

BCL-2 Antibody – Polyclonal

Name: BCL-2 Antibody; Polyclonal; MyBioSource
Brand: MyBioSource
SKU: MBS191076
Price: 465 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS191076

Bcl-2 protein suppresses apoptosis in a variety of cellular systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability and appears to function in a feedback loop… More details

$465 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Bcl-2, Polyclonal Antibody

Full Product Name

Bcl-2 (Phospho-Ser70)

Product Gene Name

anti-Bcl-2 antibody

Clonality

Polyclonal

Form/Format

100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.

Specificity

This antibody detects endogenous human Bcl-2 only when phosphorylated at serine 70.

Preparation and Storage

This antibody is stable for at least one (1) year at -20 degree C. Can be stored at 4 degree C for short-term.

Molecular Weight

22,337 Da

OMIM

M13994 mRNA

3D Structure

ModBase 3D Structure for P10415

Antigen

Peptide sequence that includes phosphorylation site of serine 70 (R-T-S(p)-P-L) derived from human Bcl-2 and conjugated to KLH.

Applications Tested/Suitable for anti-Bcl-2 antibody

Immunohistochemistry (IHC)

Application Notes for anti-Bcl-2 antibody

Immunohistochemistry: use at dilution of 1:50-1:100.
Dilute in PBS or medium that is identical to that used in the assay system.

Other Notes

Small volumes of anti-Bcl-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Bcl-2 purchase

MBS191076 is an antibody targeting Bcl-2. Also known as Bcl-2 (Phospho-Ser70).

Additional Details

NCBI/Uniprot data below describe general gene information for Bcl-2. It may not necessarily be applicable to this product.

NCBI GI #

72198189

NCBI GeneID

596

NCBI Accession #

NP_000624.2

UniProt Primary Accession #

P10415

UniProt Secondary Accession #

P10416; Q13842; Q16197; C9JHD5

UniProt Related Accession #

P10415

GenBank Nucleotide #

NM_000633.2

NCBI Official Full Name

apoptosis regulator Bcl-2 alpha isoform

NCBI Official Synonym Full Names

BCL2, apoptosis regulator

NCBI Official Symbol

BCL2

NCBI Official Synonym Symbols

Bcl-2; PPP1R50

UniProt Protein Name

Apoptosis regulator Bcl-2

Protein Family

Bcl-2/adenovirus E1B 19 kDa-interacting protein 2-like protein

UniProt Gene Name

BCL2

NCBI Protein Information

apoptosis regulator Bcl-2

NCBI Summary for Bcl-2

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

UniProt Comments for Bcl-2

Bcl-2: a antiapoptotic member of the Bcl-2 family. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Phosphorylation by JNKs may increase its antiapoptotic functions.

Protein type: Apoptosis; Autophagy; Membrane protein, integral; Oncoprotein

Chromosomal Location of Human Ortholog: 18q21.33

Cellular Component: cytoplasm; endoplasmic reticulum; membrane; mitochondrial outer membrane; mitochondrion; nuclear membrane; nucleus; pore complex

Molecular Function: BH3 domain binding; channel activity; channel inhibitor activity; identical protein binding; protease binding; protein binding; protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding; ubiquitin protein ligase binding

Biological Process: apoptosis; B cell proliferation; B cell receptor signaling pathway; defense response to virus; DNA damage response, signal transduction resulting in induction of apoptosis; endoplasmic reticulum calcium ion homeostasis; humoral immune response; induction of apoptosis via death domain receptors; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of cellular pH reduction; negative regulation of mitochondrial depolarization; negative regulation of neuron apoptosis; neuron apoptosis; positive regulation of B cell proliferation; positive regulation of cell growth; protein polyubiquitination; regulation of calcium ion transport; regulation of mitochondrial membrane permeability; regulation of mitochondrial membrane potential; regulation of protein heterodimerization activity; regulation of protein homodimerization activity; regulation of transmembrane transporter activity; release of cytochrome c from mitochondria; response to cytokine stimulus; response to DNA damage stimulus; response to drug; response to iron ion; response to nicotine; response to toxin

Research Articles on Bcl-2

1. Our findings showed that expression of Bcl-2 decreased in transition from endometrial intraepithelial neoplasia to adrenocarcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Bcl-2 antibody

Reference Product	PubMed Publications
BAX antibody	>1023 publications with Bcl-2 and BAX
CASP3 antibody	>536 publications with Bcl-2 and CASP3
MYC antibody	>344 publications with Bcl-2 and MYC
BAD antibody	>167 publications with Bcl-2 and BAD
CASP8 antibody	>148 publications with Bcl-2 and CASP8
TP53 antibody	>100 publications with Bcl-2 and TP53
BID antibody	>91 publications with Bcl-2 and BID
BNIP3 antibody	>57 publications with Bcl-2 and BNIP3
BAK1 antibody	>55 publications with Bcl-2 and BAK1
BAG1 antibody	>49 publications with Bcl-2 and BAG1

Pathways associated with anti-Bcl-2 antibody

Products by Pathway	Pathway Diagram
ATF-2 Transcription Factor Network Pathway antibodies	ATF-2 Transcription Factor Network Pathway Diagram
Activation Of BAD And Translocation To Mitochondria Pathway antibodies	Activation Of BAD And Translocation To Mitochondria Pathway Diagram
Activation Of BH3-only Proteins Pathway antibodies	Activation Of BH3-only Proteins Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram

Diseases associated with anti-Bcl-2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2172 publications with Bcl-2 and Neoplasms
Lymphoma, Non-Hodgkin Antibodies	>614 publications with Bcl-2 and Lymphoma, Non-Hodgkin
Carcinoma Antibodies	>541 publications with Bcl-2 and Carcinoma
Lymphoma, Follicular Antibodies	>332 publications with Bcl-2 and Lymphoma, Follicular
Necrosis Antibodies	>306 publications with Bcl-2 and Necrosis
Lymphoma, Large B-Cell, Diffuse Antibodies	>298 publications with Bcl-2 and Lymphoma, Large B-Cell, Diffuse
Breast Neoplasms Antibodies	>272 publications with Bcl-2 and Breast Neoplasms
Adenocarcinoma Antibodies	>210 publications with Bcl-2 and Adenocarcinoma
Drug Toxicity Antibodies	>203 publications with Bcl-2 and Drug Toxicity
Disease Progression Antibodies	>194 publications with Bcl-2 and Disease Progression

Organs/Tissues associated with anti-Bcl-2 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>825 publications with Bcl-2 and Blood
Bone Antibodies	>283 publications with Bcl-2 and Bone
Brain Antibodies	>266 publications with Bcl-2 and Brain
Muscle Antibodies	>227 publications with Bcl-2 and Muscle
Lymph Node Antibodies	>216 publications with Bcl-2 and Lymph Node
Bone Marrow Antibodies	>206 publications with Bcl-2 and Bone Marrow
Lung Antibodies	>204 publications with Bcl-2 and Lung
Lymph Antibodies	>174 publications with Bcl-2 and Lymph
Vascular Antibodies	>166 publications with Bcl-2 and Vascular
Heart Antibodies	>159 publications with Bcl-2 and Heart