Hybridoma Cloning Medium Accessory Reagent

Store at -20 degree C for up to six months.

Add BriClone Hybridoma Cloning Medium to hybridoma culture medim as a 5% (v/v) supplement. BriClone is suitable for use in the post-fusion stages of hybridoma production and when cloning hybridoma cell lines under conditions of limiting dilution. BriClone, when added to culture medium, eliminates the need for feeder cells in these types of cultures.
Hybridoma cell lines EP-16 and 7P41A were cloned by limiting dilution under conditions designed to seed 1 cell/well in 96-well plates. (A) = with 5% BriClone, (B) = with murine macrophages as feeder cells.