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Choline Acetyltransferase (CHAT) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS191355

Choline acetyltransferase (ChAT) catalyzes the transfer of an acetyl group from acetyl-CoA to choline to produce acetylcholine. ChAT is found in high concentration in the central nervous system and peripheral nervous system. Alterations in concentrations of… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Choline acetyltransferase (CHAT), Polyclonal Antibody
Full Product Name
Choline Acetyltransferase Polyclonal Antibody
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Form/Format
100ug (1mg/ml) peptide affinity-purified antibody in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Specificity
This antibody recognizes human and mouse choline acetyltransferase
Molecular Weight
74,361 Da
3D Structure
ModBase 3D Structure for P28329
Immunogen
Synthetic peptide corresponding to amino acids from the N-terminus to the mid-protein of human choline o-acetyltransferase.
Applications Tested/Suitable for anti-CHAT antibody
Immunoblot, Immunofluorescence (IF)
Application Notes for anti-CHAT antibody
Immunoblot: 1:1,000. A band of ~80kDa is detected.
IF: 1:100

Testing Data of anti-CHAT antibody

Detection of choline acetyltransferase in mouse brain lysate diluted 1:1,000.
Rabbit Anti-CHAT Polyclonal Antibody – Cat# MBS191355

Testing Data of anti-CHAT antibody

Detection of choline acetyltransferase in human neuroblastoma cell line SK-N-BE diluted 1:100. DAPI (blue) nuclear stain, Texas red actin stain, and FITC (green) choline acetyltransferase stain.
Rabbit Anti-CHAT Polyclonal Antibody, Testing Data – Cat# MBS191355

Other Notes

Small volumes of anti-CHAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHAT purchase

MBS191355 is an antibody targeting CHAT. Also known as Choline Acetyltransferase Polyclonal Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CHAT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
choline O-acetyltransferase isoform 1
NCBI Official Synonym Full Names
choline O-acetyltransferase
NCBI Official Symbol
CHAT
NCBI Official Synonym Symbols
CMS6; CMS1A; CMS1A2; CHOACTASE
UniProt Protein Name
Choline O-acetyltransferase
UniProt Gene Name
CHAT
UniProt Synonym Gene Names
CHOACTase; ChAT; Choline acetylase
NCBI Protein Information
choline O-acetyltransferase

NCBI Summary for CHAT

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
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UniProt Comments for CHAT

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CHAT antibody

 Reference Product  PubMed Publications
 NEDD9 antibody  >1 publications with CHAT and NEDD9

Organs/Tissues associated with anti-CHAT antibody

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